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针刺调控大鼠离心运动性骨骼肌损伤中Ca²⁺、Na⁺、H₂O₂动态流速

张学林^1,*, 赵倩¹, 刘爱珊¹, 段明亮¹, 丁静静¹, 王桦²

¹曲阜师范大学体育科学学院，曲阜 273165；²湖州师范学院生命科学学院，湖州 313000

摘要

本文旨在实时检测针刺对离心运动性骨骼肌损伤后Ca²⁺、Na⁺、H₂O₂动态流速的影响，探讨针刺效应始动机制及信号转换途径。324 只健康雄性Wistar 大鼠随机分成6 组：对照组、离心运动组(E)、E+针刺组(EA)、EA+瞬时受体电位(transient receptor potential, TRP) 通道阻断剂组(EAT)、EA+NADPH 氧化酶2 (nicotinamide adenine dinucleotide phosphateoxidase 2, NOX2)阻断剂组(EAN)和EA+PBS 缓冲液安慰剂组(EAP)。经皮电刺激腓肠肌离心收缩后，即刻阻断剂预处理。30 min 后，针刺内侧腓肠肌，采用非损伤微测技术实时检测留针期、拔针后即刻、3 h、6 h 和24 h 时相Ca²⁺、Na⁺、H₂O₂动态流速。结果显示：与E组相比，EA组Ca²⁺净外流显著增加(P < 0.05)，同时Na⁺净内流时相提前，伴随H₂O₂净外流大幅度下降(P < 0.05)。然而，EAT 和EAN组针刺效应显著下降，且H₂O₂过度净外流(P < 0.001)。以上结果提示，针刺通过激活TRP通道协同NOX2 活性，调控了离心运动性骨骼肌损伤后Na⁺、Ca²⁺和H₂O₂动态流速。

关键词： 离心运动; 骨骼肌损伤; 针刺; 非损伤微测技术; 机械信号转导

Acupuncture regulates dynamic flux of Ca²⁺, Na⁺, and H₂O₂ in skeletal muscle injury induced by eccentric exercise in rats

ZHANG Xue-Lin^1,*, ZHAO Qian¹, LIU Ai-Shan¹, DUAN Ming-Liang¹, DING Jing-Jing¹, WANG Hua²

¹College of Physical Education, Qufu Normal University, Qufu 273165, China；²College of Life Sciences, Huzhou Normal University, Huzhou 313000, China

Abstract

This study aimed to investigate the effects of acupuncture on dynamic changes in Ca2+, Na+, and H2O2 flux following eccentric exercise-induced muscle injury. The total of 324 healthy male Wistar rats were randomly divided into 6 groups: control group (C), eccentric exercise group (E), eccentric exercise with acupuncture group (EA), EA with TRP channel blocker group (EAT), EA with NOX2 blocker group (EAN) and EA with placebo group (EAP). Gastrocnemius muscles were subject to lengthening contractions with percutaneous electrical stimulation, followed by immediate pretreatment with blocking agents. After 30 min, acupuncture needling was administered to the gastrocnemius muscle, and real-time dynamic changes of Ca2+, Na+ and H2O2 flux were measured with non-invasive micro-test technique during the needle retention period, immediately, 3 h, 6 h, and 24 h post-extraction respectively. Results showed that compared with the E group, acupuncture significantly increased net Ca2+ efflux (P < 0.05), extended the period of net Na+ influx, and significantly decreased net H2O2 efflux (P < 0.05). However, these effects were significantly attenuated in the EAT and EAN groups, where excessive net H2O2 efflux was observed (P < 0.001). These findings indicate that acupuncture regulates the dynamic changes of Ca2+, Na+ and H2O2 flux by activating the TRP channels and interacting with NOX2 activity following eccentric exercise-induced skeletal muscle injury.

Key words: eccentric exercise; skeletal muscle injury; acupuncture; the non-invasive micro-test technique; mechanotransduction

收稿日期：　　录用日期：

通讯作者：张学林　　E-mail:

DOI: 10.13294/j.aps.2025.0019

引用本文：

张学林, 赵倩, 刘爱珊, 段明亮, 丁静静, 王桦. 针刺调控大鼠离心运动性骨骼肌损伤中Ca²⁺、Na⁺、H₂O₂动态流速[J]. 生理学报 2025; 77 (1): 47-61.

ZHANG Xue-Lin, ZHAO Qian, LIU Ai-Shan, DUAN Ming-Liang, DING Jing-Jing, WANG Hua. Acupuncture regulates dynamic flux of Ca²⁺, Na⁺, and H₂O₂ in skeletal muscle injury induced by eccentric exercise in rats. Acta Physiol Sin 2025; 77 (1): 47-61 (in Chinese with English abstract).