当期文章

基于激光散斑对比成像技术探讨高盐饮食对小鼠肾血管反应性的影响

胡莉霞, 姜绍鹏, 肖翱, 孟欣欣, 王明晓^*

遵义医科大学珠海校区生理教研室，珠海 519040

摘要

本研究旨在利用激光散斑对比成像(laser speckle contrast imaging, LSCI)技术进行在体实验，探究高盐饮食对小鼠肾血管反应性的影响。LSCI 是一种基于激光散斑原理连续监测组织血流的技术，目前主要广泛应用于记录皮肤、脑等组织微循环血流变化。肾脏位于腹膜之后，位置易受腹部器官移动的影响，在体利用LSCI测量肾血流变化的研究较少。本研究利用自制肾脏杯游离肾脏，使其位置相对固定，然后应用LSCI 技术在体观察高盐饮食(8% Na+)对雌雄小鼠肾血管反应性的影响。结果显示，短期(1 周)高盐饮食未影响雌雄小鼠尾动脉收缩压，但显著提高肾小球滤过率和肾血流量。和正常饮食组小鼠相比，高盐饮食组雄性小鼠闭塞后反应性充血(post-occlusive reactive hyperemia, PORH)比值显著降低，而雌性小鼠PORH比值没有明显变化。以上结果一方面证实短期高盐饮食虽然不会导致血压变化，但已影响肾血管反应性，且作用具有性别差异；另一方面为在体应用LSCI检测肾血管反应性、评估肾微循环功能提供一定的技术支持。

关键词： 激光散斑对比成像; 高盐饮食; 闭塞后反应性充血; 性别差异

Effects of high salt diet on renal vascular reactivity in mice detected by laser speckle contrast imaging

HU Li-Xia, JIANG Shao-Peng, XIAO Ao, MENG Xin-Xin, WANG Ming-Xiao^*

Department of Physiology, Zhuhai Campus of Zunyi Medical University, Zhuhai 519040, China

Abstract

The aim of this study was to conduct in vivo experiments using laser speckle contrast imaging (LSCI) technology to investigate the effects of high salt diet on renal vascular reactivity in mice. LSCI is a technology for monitoring blood flow based on the laser speckle principle. It has been widely used to detect microcirculatory functions in tissues such as the skin and brain. The kidneys are located behind the peritoneum, and their position is easily affected by the movement of abdominal organs. Measuring renal microcirculation in a living individual is difficult. The present study used a self-made kidney cup to isolate the kidney and fix its position relatively, and then applied LSCI technology to explore the effect of high salt diet (8% Na⁺) on renal vascular reactivity in male and female mice in vivo. The results showed that a short-term high salt diet (1 week) did not affect the systolic blood pressure of the tail artery, while significantly increased glomerular filtration rate (GFR) and renal blood flow (RBF). Compared with the normal salt diet group, the high salt diet group showed a significant decrease in the ratio of post-occlusive reactive hyperemia (PORH) in male mice, while there was no significant change in the PORH ratio in female mice. These results suggest that, although a short-term high salt diet does not cause changes in blood pressure, it has already affected renal vascular reactivity and has gender differences in its effects. Furthermore, the present study provides a basis for renal microcirculation assessment using LSCI in vivo.

Key words: laser speckle contrast imaging; high salt diet; post-occlusive reactive hyperemia; gender differences

收稿日期：　　录用日期：

通讯作者：王明晓　　E-mail:

引用本文：

胡莉霞, 姜绍鹏, 肖翱, 孟欣欣, 王明晓. 基于激光散斑对比成像技术探讨高盐饮食对小鼠肾血管反应性的影响[J]. 生理学报 2024; 76 (6): 979-986.

HU Li-Xia, JIANG Shao-Peng, XIAO Ao, MENG Xin-Xin, WANG Ming-Xiao. Effects of high salt diet on renal vascular reactivity in mice detected by laser speckle contrast imaging. Acta Physiol Sin 2024; 76 (6): 979-986 (in Chinese with English abstract).