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运动对高脂膳食大鼠肝脏血小板活化因子代谢的影响

董尚炎¹, 李伟¹, 王立丰¹, 林振茂^2,3, 陈明健^3,4, 李婷^1,3,*

¹浙江师范大学体育与健康科学学院，金华 321004；²汕头市体育运动学校，汕头 515000；³华南师范大学体育科学学院， 广州 510000；⁴佛山大学人文与教育学院，佛山 528000

摘要

本文旨在探讨高脂膳食和/或运动对肝脏血小板活化因子(platelet-activating factor, PAF)的影响及可能的机制。将32只雄性Sprague-Dawley (SD)大鼠随机分为对照组(control, C)、高脂膳食组(high-fat diet, H)、运动组(exercise, EC)和高脂膳食+运动组(high-fat diet+exercise, EH)，16周的膳食和/或运动干预后取血清测定血脂和肝损伤标记物，取肝组织采用液相色谱/质谱进行脂质组学分析，通过qPCR和Western blot测定PAF代谢相关酶的基因和蛋白表达。结果显示：(1)高脂膳食显著增加了大鼠低密度脂蛋白胆固醇(low-density lipoprotein-cholesterol, LDL-C)、以及嘌呤核苷酸磷酸化酶(purine nucleoside phosphorylase, PNP)和丙二醛(malondialdehyde, MDA)等肝损伤标记物含量，运动则降低了这些指标；(2)高脂膳食显著增加了肝脏PAF含量，运动缓解了由高脂膳食引起的这种变化；(3)高脂膳食导致PAF关键合成酶PAF-CPT蛋白表达及磷脂酶A2 (phospholipase A2, PLA2)基因和蛋白表达增加，水解酶Lp-PLA2基因和蛋白表达及PAF-AH(II)蛋白表达也增加，但合成酶增加程度显著高于水解酶；运动则增加了Lp-PLA2和PAF-AH(II)蛋白表达并减少了PAF关键合成酶PAF-CPT和PLA2的蛋白表达。综上所述，高脂膳食诱导PAF含量增加的可能机制主要是在翻译水平增加其病理性合成，而运动则通过增加PAF水解和减少其合成两方面降低高脂膳食大鼠肝脏PAF含量。

关键词： 运动; 高脂膳食; 肝脏; 血小板活化因子

The effect of exercise on platelet-activating factor metabolism in the livers of rats fed high-fat diet 

DONG Shang-Yan¹, LI Wei¹, WANG Li-Feng¹, LIN Zhen-Mao^2,3, CHEN Ming-Jian^3,4, LI Ting^1,3,*

¹College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China；²Shantou Sports School, Shantou 515000, China；³School of Physical Education and Sports Science, South China Normal University, Guangzhou 510000, China；⁴College of Humanities and Education, Foshan University, Foshan 528000, China

Abstract

This paper aimed to investigate the effects of exercise on hepatic platelet-activating factor (PAF) metabolism in rats fed a high-fat diet. Thirty-two male Sprague-Dawley (SD) rats were divided into control group (C), high-fat diet group (H), exercise group (EC), and high-fat diet+exercise group (EH). Serum lipids, glucose, insulin and markers of hepatic injury after a 16-week dietary and/or exercise intervention (60 min/day, 6 times/week) were measured by biochemical analysis; liver lipidomic profiles were analyzed by liquid chromatograph-mass spectrometer (LC-MS). Gene and protein expression of enzymes related to PAF metabolism were determined by qPCR and Western blot respectively. The results showed that high-fat diet feeding significantly increased the levels of low-density lipoprotein-cholesterol (LDL-C) and liver injury markers including purine nucleoside phosphorylase (PNP) and malondialdehyde (MDA) in rats, which were decreased by exercise. Furthermore, high-fat diet feeding significantly increased the hepatic PAF content, which was also attenuated by exercise. In addition, although high-fat diet treatment resulted in an increase in the expression of both PAF synthetase (PAF-CPT and PLA2) and hydrolase (Lp-PLA2 and PAF-AH(II)), induction of PAF synthetase was much greater than that of PAF hydrolase. While exercise increased the expression of Lp-PLA2 and PAF-AH(II) and decreased the expression of PAF-CPT and PLA2, key PAF synthesizing enzymes. In conclusion, high-fat diet-induced increase in hepatic PAF content is mainly due to the increase of its pathological synthesis at the translational level. Exercise reduces hepatic PAF content in high-fat fed rats by increasing PAF hydrolysis and decreasing its synthesis.

Key words: Exercise; high-fat diet; liver; platelet-activating factor

收稿日期：　　录用日期：

通讯作者：李婷　　E-mail:

DOI: 10.13294/j.aps.2024.0040

引用本文：

董尚炎, 李伟, 王立丰, 林振茂, 陈明健, 李婷. 运动对高脂膳食大鼠肝脏血小板活化因子代谢的影响[J]. 生理学报 2024; 76 (4): 537-546.

DONG Shang-Yan, LI Wei, WANG Li-Feng, LIN Zhen-Mao, CHEN Ming-Jian, LI Ting. The effect of exercise on platelet-activating factor metabolism in the livers of rats fed high-fat diet . Acta Physiol Sin 2024; 76 (4): 537-546 (in Chinese with English abstract).