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II组和III组mGluRs在慢性间歇性低氧诱导的大鼠颈动脉体可塑性中的作用

赵晨露, 李超红, 刘玉珍^*

新乡医学院第一附属医院，河南省神经修复重点实验室，生命科学研究中心，卫辉 453100

摘要

本文旨在探究II组和III组代谢型谷氨酸受体(metabotropic glutamate receptors, mGluRs)在慢性间歇性低氧(chronic intermittent hypoxia, CIH)所致大鼠颈动脉体可塑性中的作用。用Oxycycler A84特制低氧室对Sprague Dawley (SD)大鼠进行CIH处理4周，模型制备结束后测量大鼠尾动脉血压，用RT-qPCR检测颈动脉体mGluR2/3/8 mRNA表达水平，用离体胞外颈动脉窦神经放电记录技术检测颈动脉窦神经活性，并用急性间歇性低氧(acute intermittent hypoxia, AIH)诱导颈动脉体感觉长时程易化(sensory long-term facilitation, sLTF)，观察II组和III组mGluRs在CIH诱导的颈动脉体可塑性中的作用。结果显示，CIH处理4周后，和对照组相比，CIH组大鼠血压明显升高，颈动脉体mGluR2/3 mRNA表达水平下调，而mGluR8 mRNA表达水平上调；电生理结果显示，AIH诱导CIH组颈动脉体出现sLTF，激活II组mGluRs对CIH组大鼠颈动脉体sLTF没有影响，而激活III组mGluRs完全抑制sLTF。以上结果提示，CIH可导致大鼠血压升高，且激活III组mGluRs可抑制CIH诱导的颈动脉体可塑性。

关键词： II组mGluRs; III组mGluRs; 颈动脉体; 可塑性

Role of group II and III mGluRs in carotid body plasticity induced by chronic intermittent hypoxia in rats

ZHAO Chen-Lu, LI Chao-Hong, LIU Yu-Zhen^*

The First Affiliated Hospital of Xinxiang Medical University, Henan Key Laboratory of Neural Repairment, Life Sciences Research Center, Weihui 453100, China

Abstract

The aim of the present study was to explore the role of group II and III metabotropic glutamate receptors (mGluRs) in carotid body plasticity induced by chronic intermittent hypoxia (CIH) in rats. Sprague Dawley (SD) rats were treated with CIH in Oxycycler A84 hypoxic chamber for 4 weeks, and the tail artery blood pressure was measured at the end of model preparation. RT-qPCR was performed to examine the mRNA expression levels of mGluR2/3/8 in rat carotid body. Carotid sinus nerve activity was detected by ex vivo carotid sinus nerve discharge recording technique, and acute intermittent hypoxia (AIH) was administered to induce carotid body sensory long-term facilitation (sLTF), in order to observe the role of group II and group III mGluRs in carotid body plasticity induced by CIH. The results showed that: 1) After 4 weeks of CIH exposure, the blood pressure of rats increased significantly; 2) CIH down-regulated the mRNA levels of mGluR2/3, and up-regulated the mRNA level of mGluR8 in the carotid body; 3) AIH induced sLTF in carotid body of CIH group. In the CIH group, activation of group II mGluRs had no effect on sLTF of carotid body, while activation of group III mGluRs completely inhibited sLTF. These results suggest that CIH increases blood pressure in rats, and group III mGluRs play an inhibitory role in CIH-induced carotid body plasticity in rats.

Key words: group II mGluRs; group III mGluRs; carotid body; plasticity

收稿日期：　　录用日期：

通讯作者：刘玉珍　　E-mail: yuzhenliu@xxmu.edu.cn

引用本文：

赵晨露, 李超红, 刘玉珍. II组和III组mGluRs在慢性间歇性低氧诱导的大鼠颈动脉体可塑性中的作用[J]. 生理学报 2023; 75 (4): 529-536.

ZHAO Chen-Lu, LI Chao-Hong, LIU Yu-Zhen. Role of group II and III mGluRs in carotid body plasticity induced by chronic intermittent hypoxia in rats. Acta Physiol Sin 2023; 75 (4): 529-536 (in Chinese with English abstract).