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CircRNA-0028171调控三氧化二砷诱导的血管内皮细胞凋亡

吴继臣¹, 金赛迪², 宋佳航², 刘鑫琪², 马文俊¹, 常琳¹, 官晓翔², 张明宇², 刘嘉祺¹, 付惠¹, 王莹³, 许超千^2,*

¹牡丹江医学院药学院，牡丹江 157400；²哈尔滨医科大学药学院，哈尔滨 150081；³牡丹江医学院基础医学院，牡丹江 157400

摘要

本文旨在研究circRNA-0028171对三氧化二砷(As2O3)诱导的血管内皮细胞凋亡的调控作用。用0~15 μmol/L As2O3作用人脐静脉血管内皮细胞(human umbilical vein endothelial cells, HUVECs) 24 h，用MTT法检测HUVECs损伤情况，用实时定量PCR检测circRNA-0028171及凋亡相关蛋白Bcl-2、Bax mRNA表达水平，用Western blot检测Bcl-2、Bax蛋白表达水平。通过转染circRNA-0028171过表达质粒和siRNA观察circRNA-0028171是否参与As2O3对HUVECs的调控。结果显示，与对照组相比，As2O3组细胞活性显著下降，Bcl-2/Bax mRNA比值和蛋白比值显著降低，circRNA-0028171显著低表达。过表达circRNA-0028171抑制As2O3诱导的HUVECs凋亡，而敲减circRNA-0028171促进As2O3诱导的HUVECs凋亡。上述结果提示，circRNA-0028171参与调控As2O3诱导的血管内皮细胞凋亡过程。

关键词： 环状RNA; 血管内皮细胞; 三氧化二砷; 凋亡

CircRNA-0028171 regulates arsenic trioxide-induced apoptosis in vascular endothelial cells

WU Ji-Chen¹, JIN Sai-Di², SONG Jia-Hang², LIU Xin-Qi², MA Wen-Jun¹, CHANG Lin¹, GUAN Xiao-Xiang², ZHANG Ming-Yu², LIU Jia-Qi¹, FU Hui¹, WANG Ying³, XU Chao-Qian^2,*

¹College of Pharmacy, Mudanjiang Medical University, Mudanjiang 157400, China；²College of Pharmacy, Harbin Medical University, Harbin 150081, China；³College of Basic Medicine, Mudanjiang Medical University, Mudanjiang 157400, China

Abstract

The present study was aimed to investigate the effects of circRNA-0028171 on the apoptosis of vascular endothelial cells induced by arsenic trioxide (As2O3). Human umbilical vein endothelial cells (HUVECs) were treated with 0–15 μmol/L As2O3 for 24 h. Then, cellular viability was measured by MTT assay. The expression levels of circRNA-0028171, Bcl-2 and Bax mRNA were detected by real-time quantitative PCR. Bcl-2/Bax protein ratio was detected by Western blot. Whether circRNA-0028171 was involved in the regulation of HUVECs by As2O3 was investigated by transfection with overexpression plasmid of circRNA-0028171 and siRNA. The results showed that compared with the control group, As2O3 group showed decreased cellular viability, reduced Bcl-2/Bax mRNA and protein ratios, and significantly lower expression of circRNA-0028171. Overexpression of circRNA-0028171 inhibited apoptosis of HUVECs induced by As2O3. Knockdown of circRNA-0028171 by siRNA promoted As2O3-induced apoptosis in HUVECs. These results suggest that circRNA-0028171 is involved in the vascular endothelial cell apoptosis induced by As2O3.

Key words: circular RNAs; vascular endothelial cells; arsenic trioxide; apoptosis

收稿日期：　　录用日期：

通讯作者：许超千　　E-mail: xuchaoqian@ems.hrbmu.edu.cn

DOI: 10.13294/j.aps.2022.0047

引用本文：

吴继臣, 金赛迪, 宋佳航, 刘鑫琪, 马文俊, 常琳, 官晓翔, 张明宇, 刘嘉祺, 付惠, 王莹, 许超千. CircRNA-0028171调控三氧化二砷诱导的血管内皮细胞凋亡[J]. 生理学报 2022; 74 (5): 763-772.

WU Ji-Chen, JIN Sai-Di, SONG Jia-Hang, LIU Xin-Qi, MA Wen-Jun, CHANG Lin, GUAN Xiao-Xiang, ZHANG Ming-Yu, LIU Jia-Qi, FU Hui, WANG Ying, XU Chao-Qian. CircRNA-0028171 regulates arsenic trioxide-induced apoptosis in vascular endothelial cells. Acta Physiol Sin 2022; 74 (5): 763-772 (in Chinese with English abstract).