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高强度间歇训练对小鼠抑郁行为的作用及机制

刘家辉^1,2, 杨超³, 顾秋香¹, 梁海英⁴, 刘东^1,3, 刘军², 刘涛², 蔡成云^1,*

¹南通大学生命科学学院，南通 226001；²南通大学体育科学学院，南通 226001；³南通大学神经再生重点实验室，南通 226001；⁴福建医科大学附属龙岩市第一医院，龙岩 364000

摘要

本文旨在明确高强度间歇训练(high-intensity interval training, HIIT)对抑郁的作用及其机制。用动物跑台建立小鼠HIIT运动模型，用慢性不可预测性温和应激(chronic unpredictable mild stress, CUMS)制备抑郁模型，用行为学实验检测小鼠的抑郁相关行为。结果显示，HIIT运动可改善CUMS模型小鼠的抑郁相关行为。Western blot和ELISA结果显示，和对照组相比，CUMS模型小鼠海马、内侧前额叶皮层(medial prefrontal cortex, mPFC)、杏仁核中糖皮质激素受体(glucocorticoid receptor, GR)蛋白表达水平下调，肿瘤坏死因子α (tumor necrosis factor α, TNF-α)含量增加，而HIIT运动能够有效逆转CUMS模型小鼠的这些变化。以上结果提示，HIIT运动能够产生抗抑郁作用，这为临床治疗抑郁疾病带来新的思路和手段。

关键词： 高强度间歇运动; 抑郁; 糖皮质激素受体; 肿瘤坏死因子α

Effect of high-intensity interval exercise on depression-related behavior in mice

LIU Jia-Hui^1,2, YANG Chao³, GU Qiu-Xiang¹, LIANG Hai-Ying⁴, LIU Dong^1,3, LIU Jun², LIU Tao², CAI Cheng-Yun^1,*

¹School of Life Science, Nantong University, Nantong 226001, China；²School of Sports Science, Nantong University, Nantong 226001, China；³Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China；⁴Longyan First Hospital Affiliated to Fujian Medical University, Longyan 364000, China

Abstract

This paper was aimed to clarify the effect of high-intensity interval training (HIIT) on depression. Animal running platforms were used to establish HIIT exercise models, depression models were prepared by chronic unpredictable mild stress (CUMS), and depression-related behaviors were detected by behavioral experiments. The results showed that HIIT exercise improved depression-related behavior in CUMS model mice. Western blot and ELISA results showed that in the hippocampus, medial prefrontal cortex (mPFC) and amygdala of the CUMS model mice, glucocorticoid receptor (GR) protein expression was down-regulated, and the content of tumor necrosis factor α (TNF-α) was increased, compared with those in the control group, whereas HIIT exercise could effectively reverse these changes in CUMS model mice. These results suggest that HIIT exercise can exert antidepressant effect, which brings new ideas and means for the clinical treatment of depressive diseases.

Key words: high-intensity interval training; depression; glucocorticoid receptor; tumor necrosis factor α

收稿日期：　　录用日期：

通讯作者：蔡成云　　E-mail: caichengyun86@163.com

DOI: 10.13294/j.aps.2022.0024

引用本文：

刘家辉, 杨超, 顾秋香, 梁海英, 刘东, 刘军, 刘涛, 蔡成云. 高强度间歇训练对小鼠抑郁行为的作用及机制[J]. 生理学报 2022; 74 (2): 165-176.

LIU Jia-Hui, YANG Chao, GU Qiu-Xiang, LIANG Hai-Ying, LIU Dong, LIU Jun, LIU Tao, CAI Cheng-Yun. Effect of high-intensity interval exercise on depression-related behavior in mice. Acta Physiol Sin 2022; 74 (2): 165-176 (in Chinese with English abstract).