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大鼠海马CA3区θ~γ神经振荡与空间认知能力相关性研究

李敏^1,*, 叶政², 朱再满¹, 刘克宇³, 孙晓艳², 孟郝²

¹皖南医学院生理学教研室，芜湖 241002；²皖南医学院临床医学院，芜湖 241002；³皖南医学院研究生学院，芜湖 241002

摘要

本研究旨在探索海马CA3区θ~γ神经振荡相位-幅值耦合(phase-amplitude coupling, PAC)强度与大鼠电击回避训练前后空间辨别和认知能力改变的相关性。依据Y型迷宫电击回避训练结果，将CA3区埋置电极大鼠分为快回避反应组和普通回避反应组。无线遥测电击回避训练前后两组大鼠海马CA3区神经元局部场电位(local field potential, LFP)，利用MATLAB小波包提取分析CA3区θ振荡(3~7 Hz)与低频γ振荡(30~60 Hz) PAC变化。结果显示，快回避反应组大鼠在训练前部分频段θ~γ振荡PAC明显高于普通回避反应组(P < 0.05)。在训练“达标”后，两组大鼠均出现θ~γ振荡PAC增加(P < 0.05)，普通回避反应组大鼠部分频段θ~γ振荡PAC明显高于快回避反应组(P < 0.05, P < 0.01)。以上结果提示，一定强度的训练可以改变大鼠空间辨别和认知能力，其机制是增强大鼠海马神经元θ~γ神经振荡同步性，即提高大鼠海马神经元θ~γ相位-幅值交错频率耦合度。

关键词： 海马; θ~γ神经振荡; 相位-幅值耦合; 认知能力; 小波包提取

分类号：R338.2

Correlation between theta–gamma neural oscillations in hippocampal CA3 area and the spatial identifying and cognitive ability in rats

LI Min^1,*, YE Zheng², ZHU Zai-Man¹, LIU Ke-Yu³, SUN Xiao-Yan², MENG Hao²

¹Department of Physiology, Wannan Medical College, Wuhu 241002, China；²Clinical Medical College, Wannan Medical College, Wuhu 241002, China；³Graduate School, Wannan Medical College, Wuhu 241002, China

Abstract

The present study was aimed to explore the correlation between θ–γ neural oscillations phase-amplitude coupling (PAC) in hippocampal CA3 area and the changes of spatial identifying and cognitive ability before and after shock avoidance training in rats. According to the results of Y-type maze shock avoidance training, the rats were divided into two groups: the fast avoidance response group and the general avoidance response group. The local field potential (LFP) of hippocampal CA3 area was recorded by wireless telemetry before and after shock avoidance training. The variation of θ oscillation (3–7 Hz) and low-γ neural oscillation (30–60 Hz) PAC in hippocampal CA3 area was analyzed by MATLAB wavelet packet extraction technique. The results showed that, compared with the general avoidance response group, the fast avoidance response group exhibited higher θ–γ neural oscillation PAC in hippocampal CA3 area before training. θ–γ oscillation PAC in hippocampal CA3 area was increased in both groups after training. It was also noticed that θ–γ neural oscillation PAC of some frequency bands in the general avoidance response group were significantly higher than those in the fast avoidance response group. The results suggest that certain intensity of training can change the spatial identifying and cognitive ability of rats, and the mechanism may involve the increase of the synchrony of θ–γ neural oscillation, i.e., the enhancement of θ–γ phase-amplitude alternating frequency coupling in hippocampal neurons.

Key words: Hippocampus; theta–gamma neural oscillation; phase-amplitude coupling; cognitive ability; wavelet packet extraction

收稿日期：2021-04-13　　录用日期：2021-06-28

通讯作者：李敏　　E-mail: limin851022@163.com

DOI: 10.13294/j.aps.2021.0060

引用本文：

李敏, 叶政, 朱再满, 刘克宇, 孙晓艳, 孟郝. 大鼠海马CA3区θ~γ神经振荡与空间认知能力相关性研究[J]. 生理学报 2021; 73 (5): 855-862.

LI Min, YE Zheng, ZHU Zai-Man, LIU Ke-Yu, SUN Xiao-Yan, MENG Hao. Correlation between theta–gamma neural oscillations in hippocampal CA3 area and the spatial identifying and cognitive ability in rats. Acta Physiol Sin 2021; 73 (5): 855-862 (in Chinese with English abstract).