过刊浏览

精子发生过程中翻译后修饰的蛋白质组学研究进展

祝天喻¹, 李妍^1,2, 刘小飞¹, 齐雅玲¹, 郭雪江^1,*

¹南京医科大学生殖医学国家重点实验室，南京 211166；²南京医科大学附属逸夫医院病理与临床检验中心，南京 211166

摘要

精子发生由一系列多阶段、复杂的生物学事件所组成，受到多因素的调控。精子发生过程存在翻译延迟的现象，因 此转录和蛋白表达水平变化不完全一致。蛋白质的翻译后修饰作为蛋白质功能的重要调控方式，在精子发生过程中起着重 要调控作用。近年来，蛋白质组学(proteomics)的发展促进了蛋白质翻译后修饰的解析和功能研究。本文综述了精子发生过 程中多种翻译后修饰的蛋白质组学研究进展，并讨论了它们在精子发生、精子功能和男性生育能力中的作用以及它们在未 来临床诊疗中的价值。

关键词： 精子发生; 翻译后修饰; 蛋白质组学

分类号：R332;R363.2;R329.2

Advances in proteomic studies of post-translational modifications during spermatogenesis

ZHU Tian-Yu¹, LI Yan^1,2, LIU Xiao-Fei¹, QI Ya-Ling¹, GUO Xue-Jiang^1,*

¹State Key LaboratoryNanjing Medical University, Nanjing 211166, China of Reproductive Medicine, ；²Center of Pathology and Clinical Laboratory, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211166, China

Abstract

Spermatogenesis is composed of a series of complex biological events, which are regulated by complex factors. There is a phenomenon of delayed translation in spermatogenesis, so the changes of transcription and protein expression are not completely consistent. Thus post-translational modifications (PTMs) play a key role in spermatogenic biological events. In recent years, the development of proteomics has deepened the discovery of PTM. This paper reviews the advances in multiple PTMs proteomic during testicular spermatogenesis. Their effects on sperm function and fertility, as well as their significance for future diagnosis and treatment are discussed.

Key words: Spermatogenesis; post-translational modifications; proteomics

收稿日期：2019-07-15　　录用日期：2019-11-25

通讯作者：郭雪江　　E-mail: guo_xuejiang@njmu.edu.cn

DOI: 10.13294/j.aps.2020.0007

引用本文：

祝天喻, 李妍, 刘小飞, 齐雅玲, 郭雪江. 精子发生过程中翻译后修饰的蛋白质组学研究进展[J]. 生理学报 2020; 72 (1): 75-83.

ZHU Tian-Yu, LI Yan, LIU Xiao-Fei, QI Ya-Ling, GUO Xue-Jiang. Advances in proteomic studies of post-translational modifications during spermatogenesis. Acta Physiol Sin 2020; 72 (1): 75-83 (in Chinese with English abstract).