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小鼠外侧丘系背核上行输入对下丘神经元反应的可塑性影响

杨丹丹, 郑维维, 赵莎莎, 罗峰, 王欣^*

华中师范大学生命科学学院，湖北省遗传调控与整合生物学重点实验室，武汉 430079

摘要

听觉系统具备随着环境的变化来改变自身结构和功能的能力，这种能力称为听觉可塑性。中脑下丘(inferior colliculus, IC)是听觉系统重要的中继站，外侧丘系背核(dorsal nuclei of lateral lemniscus, DNLL)上行输入对IC神经元的声反应特性具有重要的定型作用。本研究旨在探讨DNLL上行输入在IC神经元可塑性形成中的作用。以昆明小鼠为实验动物，持续电刺激对侧DNLL，采用单单位细胞外记录的方法观察IC神经元的可塑性变化。结果显示，95%的受抑制IC神经元和86%的受易化IC神经元在对侧DNLL 30 min电刺激停止后仍表现出可塑性的变化。在对侧DNLL电刺激停止1 h后，74%的受抑制IC神经元发生的可塑性变化消失，26%的受抑制IC神经元发生的可塑性变化仍然存在。以上结果提示，对侧DNLL上行输入可引起IC神经元的可塑性变化，并维持较长时间，这有利于提高IC神经元的声信号检测能力。

关键词： 外侧丘系背核; 上行输入; 听觉可塑性; 下丘神经元; 小鼠

分类号：Q6；R338

Ascending input from dorsal nuclei of lateral lemniscus mediates the plasticity of inferior colliculus neurons in mice

YANG Dan-Dan, ZHENG Wei-Wei, ZHAO Sha-Sha, LUO Feng, WANG Xin^*

School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, China

Abstract

The auditory system has the ability to adjust its structure and function as the environment changes, which is called auditory plasticity. In the auditory system, inferior colliculus (IC) is an important relay station, which accepts the ascending inputs from dorsal nuclei of lateral lemniscus (DNLL). The present study was aimed to investigate the role of the DNLL in the formation of the plasticity of IC neurons. Here, we used extracellular single unit recording and electrical stimulation to investigate the plasticity of IC neurons in Kunming mice. The results showed that after the cessation of 30-minute electrical stimulation on contralateral DNLL, 95% of the inhibited IC neurons and 86% of the facilitated IC neurons showed plastic changes. Moreover, 1 h after the contralateral DNLL stimulation was stopped, the plastic changes in 74% of the inhibited IC neurons vanished, but still were maintained in 26% of the inhibited IC neurons. These results suggest that the contralateral DNLL ascending input can induce plastic changes of IC neurons, and this kind of effect can be maintained for a certain period of time, which is beneficial to enhance the sound intensity sensitivity of IC neurons.

Key words: dorsal nuclei of lateral lemniscus; ascending input; auditory plasticity; inferior colliculus; mouse

收稿日期：2017-06-28　　录用日期：2018-03-28

通讯作者：王欣　　E-mail: 675341542@qq.com

DOI: 10.13294/j.aps.2018.0028

引用本文：

杨丹丹, 郑维维, 赵莎莎, 罗峰, 王欣. 小鼠外侧丘系背核上行输入对下丘神经元反应的可塑性影响[J]. 生理学报 2018; 70 (2): 132-140.

YANG Dan-Dan, ZHENG Wei-Wei, ZHAO Sha-Sha, LUO Feng, WANG Xin. Ascending input from dorsal nuclei of lateral lemniscus mediates the plasticity of inferior colliculus neurons in mice. Acta Physiol Sin 2018; 70 (2): 132-140 (in Chinese with English abstract).