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实验时间对老年Sprague-Dawley 大鼠认知行为的影响

邓世宁, 司文, 孙丽娟, 曾庆文^*, 曹晓华^*

华东师范大学脑功能基因组学教育部重点实验室，上海市脑功能基因组学重点实验室，上海200062

摘要

为了探明老年Sprague-Dawley (SD)大鼠的学习记忆能力是否受到实验时间的影响，我们将老年SD 大鼠随机分为两组：一组在上午(8:00 ~ 11:00)进行行为学实验(上午组，AM 组)，另一组则在下午(15:00~18:00)进行行为学实验(下午组，PM 组)。结果显示，在开场实验中，PM 组的活动量显著高于AM 组；此外，在Morr is 水迷宫任务的训练阶段，PM 组比AM 组表现出更好的空间学习记忆能力，而且在检测(probe-test)过程中，PM 组也表现出更强的空间记忆能力；而在新异个体识别(social discrimination)行为实验中，两组大鼠没有表现出显著性差异。以上结果表明，实验时间是老年SD 大鼠在水迷宫任务中表现的影响因素，但并不是新异个体识别任务的影响因素。提示实验时间对老年大鼠不同的学习记忆任务具有不同的影响。

关键词： 老年大鼠; 学习记忆; 开场实验; Mo r r i s 水迷宫; 新异个体识别

分类号：Q427

[Experiment-time affects the cognitive behavior in aged Sprague-Dawley rats.] [Ariticle in Chinese]

DENG Shi-Ning, SI Wen, SUN Li-Juan, ZENG Qing-Wen^*, CAO Xiao-Hua^*

Shanghai Key Laboratory of Brain Functional Genomics, Key Laboratory of Brain Functional Genomics of Ministry of Education, East China Normal University, Shanghai 200062, China

Abstract

In order to investigate if the experiment-time affects cognitive performance in aged rats in different learning and memorybehavioral tests, the aged Sprague-Dawley (SD) rats were divided into two groups randomly and subjected to open field test, Morriswater maze test and social discrimination test. The experiment of one group was conducted in 8:00 - 11:00 AM (AM group), and theother group in 15:00 - 18:00 PM (PM group). PM group exhibited higher locomotor activity than AM group in the open field test.Compared with AM group rats, PM group rats had significantly shorter swimming distance and escape latency to find the platform inMorris water maze training session, but no significant difference in the swimming velocity was observed between the two groups. Andin probe-test, PM group spent more time in target quadrant than AM group. These results revealed that PM group showed betterspatial learning and memory abilities than AM group. However, there was no significant difference between the two groups in socialinvestigation index in social discrimination test. These results show that experiment-time may affect cognitive performance in Morriswater maze but not in social discrimination task in aged rats. And the results indicate that experiment-time affects cognitive performanceof aged rats selectively in different learning and memory behavioral tasks.

Key words: aged rats; learning and memory; open field; Morris water maze; social discrimination

收稿日期：2010-02-23　　录用日期：2010-03-26

通讯作者：曾庆文,曹晓华　　E-mail: qwzeng@brain.ecnu.edu.cn,xhcao@brain.ecnu.edu.cn

引用本文：

邓世宁, 司文, 孙丽娟, 曾庆文, 曹晓华. 实验时间对老年Sprague-Dawley 大鼠认知行为的影响[J]. 生理学报 2010; 62 (3): 231-236.

DENG Shi-Ning, SI Wen, SUN Li-Juan, ZENG Qing-Wen, CAO Xiao-Hua. [Experiment-time affects the cognitive behavior in aged Sprague-Dawley rats.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (3): 231-236 (in Chinese with English abstract).