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Omega-3 鱼油对铅暴露大鼠海马齿状回长时程增强损伤的保护作用

曹秀菁, 曹杰杰, 陈婷婷, 陈伟恒, 阮迪云

安徽医科大学公共卫生学院，合肥230032； 中国科技大学生命科学学院，合肥 230027

摘要

本文旨在探讨每天灌胃omega-3 鱼油对慢性铅暴露(0.2% 醋酸铅)导致的大鼠海马齿状回(dentate gyrus, DG)区突触可塑性损伤有无保护作用。将新生Wistar 大鼠分为4 组：正常对照组、铅组、正常+ 鱼油组、铅+ 鱼油组，染铅方式为饮用0.2% 醋酸铅水，鱼油按0.4 mL/100 g 体重采用灌胃方式给药，至大鼠80~90 d 时，4 组大鼠进行海马DG 区的场电位记录，记录兴奋性突触后电位(excitatory postsynaptic potential, EPSP)、群峰电位(population spike, PS)的长时程增强(long-term potentiation,LTP)。结果显示：出生后的铅暴露损伤了大鼠海马DG 区的LTP；鱼油对正常对照组的LTP 无显著影响，而对染铅大鼠LTP的损伤有明显的修复作用。以上结果提示omega-3 鱼油可能对慢性铅暴露导致的LTP 损害具有一定的保护作用。

关键词： 铅; 鱼油; 长时程增强; 海马

分类号：Q6; R15

Protective effects of omega-3 fish oil on lead-induced impairment of long-term potentiation in rat dentate gyrus in vivo

CAO Xiu-Jing, CAO Jie-Jie, CHEN Ting-Ting, CHEN Wei-Heng, RUAN Di-Yun

School of Public Health, Anhui Medical University, Hefei 230032, China； School of Life Science, University of Science and Technology of China, Hefei 230027, China

Abstract

In order to evaluate the effect of omega-3 fish oil supplement by gavage (0.4 mL/100 g body weight) on the chronic leadinduced(0.2% lead acetate) impairments of long-term potentiation (LTP) in rat dentate gyrus (DG) in vivo, we designed the experimentswhich were carried out in four groups of newborn Wistar rats (the control, the lead-exposed, the control with fish oil treatmentand the lead-exposed with fish oil treatment, respectively). The excitatory postsynaptic potential (EPSP) and population spike (PS)amplitude were measured in the DG of rats with above different treatments at the age of 80-90 d in response to stimulation applied tothe lateral perforant path. The results showed (1) postnatal chronic lead-exposure impaired LTP measured on both EPSP slope and PSamplitude in DG area of the hippocampus; (2) in the control rats, omega-3 fish oil had no effect on LTP while in the lead-exposed rats,omega-3 fish oil had a protective effect on LTP. These results suggest that omega-3 fish oil supplement could protect rats from the leadinducedimpairment of LTP. Omega-3 fish oil might be a preventive substance in reducing LTP deficits induced by lead.

Key words: lead; fish oil; long-term potentiation; hippocampus

收稿日期：2010-03-09　　录用日期：2010-05-20

通讯作者：曹秀菁　　E-mail: xiujingcao@yeah.net

引用本文：

曹秀菁, 曹杰杰, 陈婷婷, 陈伟恒, 阮迪云. Omega-3 鱼油对铅暴露大鼠海马齿状回长时程增强损伤的保护作用[J]. 生理学报 2010; 62 (3): 225-230.

CAO Xiu-Jing, CAO Jie-Jie, CHEN Ting-Ting, CHEN Wei-Heng, RUAN Di-Yun. Protective effects of omega-3 fish oil on lead-induced impairment of long-term potentiation in rat dentate gyrus in vivo. Acta Physiol Sin 2010; 62 (3): 225-230 (in Chinese with English abstract).