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雌性棕色田鼠和昆明小鼠在慢性间歇性低氧条件下血象变化的比较

刘彬, 王振龙, 路纪琪^*, 杨艳艳

郑州大学生物多样性与生态学研究所，郑州 450001

摘要

本文旨在对雌性棕色田鼠(Lasiopodomys mandarinus)和昆明小鼠(Mus musculus)在慢性间歇性低氧条件下血象的变化进行比较，从而为地下生活鼠种血液系统对慢性间歇性低氧的应答对策的研究提供基础数据。采用实验氧舱对棕色田鼠和昆明小鼠进行每天4 h、持续28 d 的慢性间歇性低氧(氧浓度10.0%)处理，然后对其血象进行了比较分析。结果显示：(1)常氧下棕色田鼠的红细胞计数(red blood cell count, RBC)显著高于昆明小鼠，而红细胞平均体积(mean corpuscular volume,MCV)则显著小于昆明小鼠，慢性间歇性低氧处理后昆明小鼠的MCV 和红细胞压积(hematocrit, HCT)显著增加，而棕色田鼠MCV 和HCT 无明显影响；(2)常氧条件下棕色田鼠的平均血红蛋白含量(mean corpuscular hemoglobin, MCH)显著低于昆明小鼠，而平均血红蛋白浓度(mean corpuscular hemoglobin concentration, MCHC)则显著高于昆明小鼠，慢性低氧处理后昆明小鼠血红蛋白(hemoglobin, HGB)含量和MCH 均显著增加，而在棕色田鼠仅出现MCH 的明显增加；(3)常氧条件下棕色田鼠的白细胞计数(white blood cell count, WBC)和血小板(platelet, PLT)都显著低于昆明小鼠，慢性低氧处理后棕色田鼠和昆明小鼠的WBC 和PLT 均未发生显著变化。以上结果表明，棕色田鼠作为地下生活鼠种，对低氧环境具有较好的耐受性，其血液系统对低氧胁迫的应答机制与地面生活鼠种昆明小鼠不同。

关键词： 慢性间歇性低氧; 棕色田鼠; 昆明小鼠; 血象

分类号：Q461

[Comparison of hemogram changes under chronic intermittent hypoxia in Lasiopodomys mandarinus and Kunming Mus musculus.] [Ariticle in Chinese]

Liu Bin, WANG Zhen-Long, LU Ji-Qi^*, YANG Yan-Yan

Institute of Biodiversity and Ecology, Zhengzhou University, Zhengzhou 450001, China

Abstract

Mandarin vole (Lasiopodomys mandarinus) spends almost all of its life underground and must have evolved remarkableadaptations to cope with the subterranean hypoxic stress. The aim of present study is to explore the adaptation mechanism through thecomparison of hemogram changes under chronic intermittent hypoxia in Mandarin vole and Kunming (KM) mouse (Mus musculus).Mandarin vole and KM mouse were treated with chronic intermittent hypoxia (10.0% oxygen), which was maintained by an oxygencabin, for 4 h per day during four weeks. Then blood samples from the animals with and without hypoxia treatment were analyzed bya hematology analyzer. The results showed that under normoxic condition mean corpuscular volume (MCV), mean corpuscularhemoglobin (MCH), white blood cell count (WBC) and platelet (PLT) in Mandarin vole were significantly lower than those in KMmouse. On the contrast, red blood cell count (RBC) and mean corpuscular hemoglobin concentration (MCHC) in Mandarin vole werehigher than that in KM mouse. After four-week chronic intermittent hypoxia treatment, the hemogram changes were as following. MCVand HCT were elevated in Mandarin vole, not affected in KM mouse. Both hemoglobin (HGB) content and MCH in KM mouseincreased, while only MCH increased in Mandarin vole. No obvious changes of WBC and PLT were found in two species. These resultssuggest that the adaptive mechanism of blood system in Mandarin vole responding to hypoxic conditions is different from that of KM mouse. As a subterranean vole, the Mandarin vole has a better tolerance to hypoxia.

Key words: chronic intermittent hypoxia; Mandarin vole; Kunming mouse; hemogram

收稿日期：2009-09-30　　录用日期：2010-03-11

通讯作者：路纪琪　　E-mail: lujq@zzu.edu.cn

引用本文：

刘彬, 王振龙, 路纪琪, 杨艳艳. 雌性棕色田鼠和昆明小鼠在慢性间歇性低氧条件下血象变化的比较[J]. 生理学报 2010; 62 (2): 137-142.

Liu Bin, WANG Zhen-Long, LU Ji-Qi, YANG Yan-Yan. [Comparison of hemogram changes under chronic intermittent hypoxia in Lasiopodomys mandarinus and Kunming Mus musculus.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (2): 137-142 (in Chinese with English abstract).