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移植胎鼠间充质干细胞的抗衰老作用

李军, 张洹, 刘革修^*

暨南大学医学院血液病研究所，广州 510632

摘要

本文旨在研究BALB/c 小鼠胎鼠源干细胞同种异体移植的抗衰老作用。采用无菌剖宫产取胎鼠，密度梯度离心法分离干细胞，贴壁培养法纯化扩增间充质干细胞，连续3 次尾静脉注射P1 代细胞植入15 月龄雌性BALB/c 小鼠体内。超声检查小鼠心脏，检查血清总超氧化物歧化酶活力、丙二醛含量、谷胱甘肽过氧化物酶活力，各器官做解剖学检查以及组织学衰老程度比较、评分。结果显示，Y 染色体原位杂交实验检测到移植后干细胞长期存活，移植后移植组小鼠存活日期明显长于对照组，移植3 个月后评价心功能的各指标，心脏质量指数、脾脏质量指数，心脏、肾脏、肺脏、皮肤、结肠等器官组织学衰老程度评分结果以及血液生化指标，皆优于对照组(均P<0.05)。以上结果提示，移植小鼠胎鼠源干细胞能有效地延缓小鼠衰老进程。

关键词： 胎鼠; 干细胞; 移植; 抗衰老

分类号：R363

[Anti-aging effect of transplantation of mouse fetus-derived mesenchymal stem cells.] [Ariticle in Chinese]

Li Jun, ZHANG Yuan, LIU Ge-Xiu^*

Institute of Hematonosis, Medical School, Jinan University, Guangzhou 510632, China

Abstract

To determine the role of allogeneil graft of mesenchymal stem cells in mammalian longevity, mesenchymal stem cells wereisolated from BALB/c mouse uterine-incision delivery fetus by two successive density gradient centrifugations, and then were purifiedand amplified by adherent culture. Identified P1 mesenchymal stem cells were injected (i.v.) through vena caudalis into the 15-montholdfemale BALB/c mice three times. The mice were evaluated with ultrasoundcardiogram, autopsy, score of cardiac, skin, lung, kidney,colon histopathology and serum total superoxide dismutase activity, maleic dialdehyde content, glutathione peroxidase activity. Theresults showed that after transplantation, the long-term surviving stem cells were found to be located in many organ tissues with in situY chromosomal hybridization dyeing. Median life span was increased in these animals after transplantation. Skin, cardiac, lung, kidneyand colon pathology development were delayed. The retrogradation of heart function was attenuated, the increase of heart mass index(the mass of heart/the mass of the body), and serum maleic dialdehyde content, the decrease of spleen mass index (the mass of spleen/the mass of the body), serum total superoxide dismutase activity and glutathione peroxidase activity were reduced three months aftertransplantation (all P<0.05). These results support the idea that longevity can be enhanced by transplantation of mesenchymal stemcells and reinforce the hypothesis of mesenchymal stem cell as antiager.

Key words: mouse fetus; stem cell; transplantation; anti-aging

收稿日期：2009-10-09　　录用日期：2009-11-28

通讯作者：刘革修　　E-mail: tliugx@jnu.edu.cn

引用本文：

李军, 张洹, 刘革修. 移植胎鼠间充质干细胞的抗衰老作用[J]. 生理学报 2010; 62 (1): 79-85.

Li Jun, ZHANG Yuan, LIU Ge-Xiu. [Anti-aging effect of transplantation of mouse fetus-derived mesenchymal stem cells.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (1): 79-85 (in Chinese with English abstract).