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急性低血压对清醒大鼠前庭内侧核区γ- 氨基丁酸和甘氨酸含量的影响

李香兰, 安英, 金清华, 金元哲

延边大学基础医学院生理学与病理生理学教研部，长白山生物资源与功能分子教育部重点实验室( 延边大学) ，延吉133000； 吉林医药学院机能学实验中心，吉林 132013

摘要

为了探讨急性低血压通过外周前庭器官影响前庭神经核活动的部分神经化学机制，本实验利用脑部微量透析法和高效液相色谱法，观察静脉注射硝普钠(sodium nitroprusside, SNP)诱发急性低血压时，清醒大鼠前庭内侧核(medial vestibularnucleus, MVN)内γ- 氨基丁酸(γ-aminobutyric acid, GABA)和甘氨酸(glycine, Gly)含量的变化。实验选用Wistar 雄性清洁级大鼠72 只，体重在(250±50) g，随机分为对照组、前庭器官损伤同侧组和前庭器官损伤对侧组。实验观察到，静脉注射SNP 使血压平均下降30% 时，MVN 内GABA 和Gly 含量明显减少(P<0.01)。用对氨基苯胂酸盐破坏单侧外周前庭器官两周后，静脉注射SNP 使血压降低30% 后，单侧前庭器官破坏同侧MVN 内GABA 和Gly 含量无明显变化；而单侧前庭器官破坏对侧MVN 内GABA 和Gly 含量明显减少(P<0.01)。上述结果提示，清醒动物诱发急性低血压影响MVN 功能活动过程中可能有GABA 和Gly 的参与。

关键词： 低血压; 前庭内侧核; γ - 氨基丁酸; 甘氨酸

分类号：Q463；R338.2

[Changes of γ-aminobutyric acid and glycine released in the medial vestibularnucleus following acute hypotension in conscious rats.] [Ariticle in Chinese]

LI Xiang-Lan, AN Ying, JIN Qing-Hua, Jin Yuan-Zhe

Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Key Laboratory of Natural ResourcesChangbai Mountain and Functional Molecules (Yanbian University), the State Educational Ministry of China, Yanji 133000, China；2Teaching Center of Functional Experimental Science, Jilin Medical College, Jilin 132013, China

Abstract

To understand whether some amino acids in the medial vestibular nucleus (MVN) of conscious rats are involved in theregulation of blood pressure, microdialysis technique and high performance liquid chromatography (HPLC) were used to measure thechanges of γ-aminobutyric acid (GABA) and glycine (Gly) in this central area. Wistar rats (250-350 g) were randomly divided into threeexperimental groups: the control group with intact labyrinths; the ipsilateral MVN of unilateral labyrinthectomy (UL); contralateralMVN of the UL. Acute hypotension was induced by intravenous infusion of sodium nitroprusside (SNP). Unilateral chemicallabyrinthectomy was performed 14 days before the start of the experiment to eliminate afferent signals from the peripheral vestibularreceptors in the inner ear. Blood pressure decreased by 30% after SNP injection. In the control group, GABA and Gly release reducedto 43.53%±6.58% (P<0.01) and 62.24%±7.51% (P<0.01) respectively in the MVN following SNP-induced acute hypotension inconscious rats. In the contralateral MVN of UL, GABA and Gly release also reduced to 45.85%±17.27% (P<0.01) and 73.30%±3.00%(P<0.01) respectively following SNP-induced acute hypotension in conscious rats. In contrast, in the ipsilateral MVN of UL, GABAand Gly releases were not changed following SNP-induced acute hypotension in conscious rats. These results suggest that the SNPinduced acute hypotension may influence the activity of the neurons in the MVN by the afferent impulses from the peripheralvestibular organ, and that GABA and Gly may be involved in this process.

Key words: hypotension; medial vestibular nucleus; γ-aminobutyric acid; glycine

收稿日期：2009-12-09　　录用日期：2009-12-31

通讯作者：金元哲　　E-mail: y-z-jin@ybu.edu.cn

引用本文：

李香兰, 安英, 金清华, 金元哲. 急性低血压对清醒大鼠前庭内侧核区γ- 氨基丁酸和甘氨酸含量的影响[J]. 生理学报 2010; 62 (1): 30-34.

LI Xiang-Lan, AN Ying, JIN Qing-Hua, Jin Yuan-Zhe. [Changes of γ-aminobutyric acid and glycine released in the medial vestibularnucleus following acute hypotension in conscious rats.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (1): 30-34 (in Chinese with English abstract).