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丁酸钠对抗内毒素血症小鼠肺损伤的保护作用

曾韬, 张力

Medical College, Jingchu University of Technology, Jingmen 448000, China； Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China

摘要

本文旨在研究丁酸钠对内毒素血症小鼠体内炎症反应、肺组织损伤及生存率的影响。Balb/c小鼠经腹腔注射丁酸钠(200 mg/kg)后，予以致死剂量脂多糖(20 mg/kg, i.p.)以诱导严重内毒素血症。造模后观察小鼠存活时间或在18 h后处死小鼠，收集血浆检测细胞因子TNF-α和IL-6水平；取肺组织检测肺组织湿/干重比、髓过氧化物酶活性，并经石蜡切片HE染色观察组织病理学改变。结果显示，丁酸钠明显减轻内毒素血症小鼠肺组织病理学损伤，降低肺组织湿/干重比及髓过氧化物酶活性。丁酸钠还可显著降低内毒素血症小鼠血浆TNF-α和IL-6水平，并提高实验动物生存率。以上结果提示，丁酸钠可有效抑制内毒素血症小鼠肺内炎症反应，提高内毒素血症小鼠生存率。

关键词： 组蛋白去乙酰化酶; 内毒素血症; 肺损伤; 炎症

分类号：R364.5

[Protective effects of sodium butyrate against lung injury in mice with endotoxemia.] [Article in Chinese]

ZENG Tao, ZHANG Li

Medical College, Jingchu University of Technology, Jingmen 448000, China； Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China

Abstract

The aim of the present study was to investigate the effects of sodium butyrate (SB) on systemic inflammation, lung injury and survival rate of mice with endotoxemia. Balb/c mice were pre-treated with SB or vehicle, and then endotoxemia was induced by lethal dose of lipopolysaccharide (LPS, 20 mg/kg, i.p.) and the survival rate of mice was monitored. A separated set of animals were sacrificed at 18 h after LPS challenge, and blood samples were harvested for measuring TNF-α and IL-6 levels. Lung tissues were also harvested to determine the ratio of wet weight to dry weight of lung tissue and myeloperoxidase (MPO) activity in lung tissue. In addition, the formalin-fixed lung specimens were stained with HE routinely for morphologic evaluation. The results showed that pre-treatment with SB alleviated LPS-induced morphological damage in lung tissue. This was accompanied by reduced ratio of wet weight to dry weight of lung tissue and MPO activity in lung homogenates. Additionally, the up-regulation of pro-inflammatory cytokines TNF-α and IL-6 was also suppressed by SB, while the survival rate of mice with lethal endotoxemia was significantly increased by SB pre-treatment. The results suggest that SB effectively attenuates intrapulmonary inflammatory response and improves the survival of endotoxemic mice.

Key words: histone deacetylase; endotoxemia; lung injury; inflammation

收稿日期：2011-11-06　　录用日期：2012-01-05

通讯作者：张力　　E-mail: zhangli@cqmu.edu.cn

引用本文：

曾韬, 张力. 丁酸钠对抗内毒素血症小鼠肺损伤的保护作用[J]. 生理学报 2012; 64 (3): 308-312.

ZENG Tao, ZHANG Li. [Protective effects of sodium butyrate against lung injury in mice with endotoxemia.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (3): 308-312 (in Chinese with English abstract).