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肾素-血管紧张素系统在卡巴胆碱调节胎羊心血管功能中的作用

耿春松, 万镇, 冯亚红, 樊一笋^*

上海交通大学医学院苏州九龙医院 检验科；妇产科，苏州 215021；南通大学建湖医院心内科，南通 224700

摘要

本研究通过静脉给予胎羊胆碱能激动剂卡巴胆碱，探讨肾素-血管紧张素系统在卡巴胆碱调节胎羊心血管功能中的作用。胎羊静脉注射胆碱能激动剂卡巴胆碱(5 μg/kg)，动态检测和分析心血管功能指标变化。在给药前后收集胎羊血液标本，放射免疫法测定血浆血管紧张素I (angiotensin I, Ang I)、血管紧张素II (angiotensin II, Ang II)、血管升压素(vasopressin, VP)水平，并测定胎羊血气、电解质浓度及血浆渗透压等指标。结果显示：胎羊静脉注射卡巴胆碱后，血压先短暂下降后又升高，在血压短暂下降的同时伴有心率减慢(P < 0.05)，并于30 min恢复到基础水平；血浆VP水平未发生明显变化(P > 0.05)，而Ang I及Ang II含量增加(P < 0.05)；胎羊静脉注射卡巴胆碱后，胎羊血气指标、电解质浓度及血浆渗透压均无明显变化(P > 0.05)。胎羊静脉给予M受体阻断剂(阿托品)预处理组(阿托品+卡巴胆碱组)血浆Ang I及Ang II含量低于未给予M受体阻断剂处理组(卡巴胆碱组)(P < 0.05)。以上结果提示，胎羊静脉注射卡巴胆碱可引起心血管功能(如血压和心率)的变化，这一反应可能与胎羊外周血液中肾素-血管紧张素系统相关激素改变有关。

关键词： 血压; 胎羊; 卡巴胆碱; 肾素-血管紧张素系统

分类号：R339

[Role of rennin-angiotensin system in cholinergic agonist carbachol-induced cardiovascular responses in ovine fetus.] [Article in Chinese]

GENG Chun-Song, WAN Zhen, FENG Ya-Hong, FAN Yi-Sun^*

Department of Clinical Laboratory； Department of Obsterics and Gynecology, Suzhou Kowloon Hospital, Shanghai Jiao Tong University Medical School, Suzhou 215021, China； Department of Cardiology, Jianhu Hospital, Nantong University, Nantong 224700, China

Abstract

To investigate the mechanisms underlying the cholinergic agonist carbachol-induced cardiovascular responses, changes of renin-angiotensin system were examined in fetal hormonal systems. In the ovine fetal model under stressless condition, the cardiovascular function was recorded. Blood samples were collected before (during baseline period) and after the intravenous administration of carbachol. Simultaneously, the levels of angiotensin I (Ang I), angiotensin II (Ang II) and vasopressin in the fetal plasma were detected by immunoradiological method. Also, blood gas, plasma osmolality and electrolyte concentrations were analyzed in blood samples. Results showed that in chronically prepared ovine fetus, intravenous infusion of carbachol led to a significant decrease of heart rate (P < 0.05), and a transient decrease followed by an increase of blood pressure (P < 0.05) within 30 min. After the intravenous infusion of carbachol, blood concentrations of Ang I and Ang II in near-term ovine fetus were both significantly increased (P < 0.05); however, blood concentration of vasopressin, values of blood gas, electrolytes and plasma osmolality in near-term ovine fetus were not significantly changed (P > 0.05). Blood levels of Ang I and Ang II in the atropine (M receptor antagonist) + carbachol intravenous administration group was lower than those in the carbachol group without atropine administration (P < 0.05). In conclusion, this study indicates that the near-term changes of cardiovascular system induced by intravenous administration of carbachol in ovine fetus, such as blood pressure and heart rate, are associated with the changes of hormones of circulatory renin-angiotensin system.

Key words: Blood pressure; ovine fetus; carbachol; renin-angiotensin system

收稿日期：2011-08-18　　录用日期：2012-04-12

通讯作者：樊一笋　　E-mail: fanyisun@hotmail.com

引用本文：

耿春松, 万镇, 冯亚红, 樊一笋. 肾素-血管紧张素系统在卡巴胆碱调节胎羊心血管功能中的作用[J]. 生理学报 2012; 64 (3): 303-307.

GENG Chun-Song, WAN Zhen, FENG Ya-Hong, FAN Yi-Sun. [Role of rennin-angiotensin system in cholinergic agonist carbachol-induced cardiovascular responses in ovine fetus.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (3): 303-307 (in Chinese with English abstract).