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可溶性endoglin减弱人早孕细胞滋养细胞的浸润功能

董微, 许群星, 韩玉环, 尹利荣

天津医科大学第二医院产科， 天津 300211

摘要

本研究旨在探讨可溶性endoglin (soluble endoglin, sEng)对人早孕细胞滋养细胞活力和浸润功能的影响。采用胰蛋白酶-DNase消化法培养人早孕期(孕6~8周)细胞滋养细胞，传代后待细胞长满至70%~80%，分别加入没有添加(对照组)和添加sEng (10 μg/L)的细胞培养液培养24 h；Transwell技术检测细胞滋养细胞的浸润功能；RT-PCR法检测细胞滋养细胞基质金属蛋白酶2 (matrix metalloproteinase-2, MMP-2)、基质金属蛋白酶9 (MMP-9) mRNA的表达；Western blot方法分别检测细胞滋养细胞MMP-2、MMP-9蛋白的表达。结果显示，sEng组细胞滋养细胞浸润能力低于对照组。与对照组比较，sEng组细胞滋养细胞MMP-2和MMP-9 mRNA及蛋白的表达明显下降(P < 0.05)。以上结果提示，sEng可能通过调节人早孕细胞滋养细胞中MMP-2、MMP-9的表达而影响细胞的浸润能力，从而参与子痫前期的发生。

关键词： 子痫前期; 细胞滋养细胞; 基质金属蛋白酶

分类号：R714.58

[Inhibitory effects of soluble endoglin on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy.] [Article in Chinese]

DONG Wei, XU Qun-Xing, HAN Yu-Huan, YIN Li-Rong

Department of Obstetrics, the Second Hospital of Tianjin Medical University, Tianjin 300211, China

Abstract

The present study aims to investigate the effects of soluble endoglin (sEng) on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy. Cytotrophoblasts of normal 6 to 8-week pregnancy were cultured by trypsin digestion method, and were incubated with cell culture medium without (control group) and with 10 μg/L sEng (sEng group), respectively for 24 h. The invasive ability was determined by transwell invasion assay, and expressions of MMP-2, MMP-9 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. The results showed that the invasive ability of cytotrophoblasts in sEng group was lower than that in control group (P < 0.05). Compared with control group, the expressions of MMP-2 and MMP-9 mRNA and protein of cytotrophoblasts were significantly lower (P < 0.05). In conclusion, sEng may participate in the genesis of preeclampsia by affecting the invasive ability of cytotrophoblasts through regulation of the expression of MMP-2 and MMP-9.

Key words: preeclampsia; cytotrophoblast; matrix metalloproteinases

收稿日期：2010-12-17　　录用日期：2011-03-14

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引用本文：

董微, 许群星, 韩玉环, 尹利荣 . 可溶性endoglin减弱人早孕细胞滋养细胞的浸润功能[J]. 生理学报 2011; 63 (3): 267-271.

DONG Wei, XU Qun-Xing, HAN Yu-Huan, YIN Li-Rong . [Inhibitory effects of soluble endoglin on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (3): 267-271 (in Chinese with English abstract).