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白藜芦醇甙减轻缺血/ 再灌注诱发的大鼠心肌细胞凋亡

张利萍, 马会杰, 布会敏, 王妹玲, 李茜, 齐钊, 张翼^*

河北医科大学生理学教研室，石家庄 050017

摘要

本研究旨在探讨白藜芦醇甙(polydatin)对大鼠缺血/ 再灌注心肌细胞凋亡的影响及其相关机制。成年雄性Sprague-Dawley 大鼠随机分为三组：对照(CON)组，缺血/ 再灌注(ischemia/reperfusion, I/R)组，白藜芦醇甙(POLY, 50 μmol/L)组。应用Langendorff 灌注技术，给予心脏30 min 缺血/60 min 再灌注，通过TUNEL 和流式细胞技术检测细胞凋亡以及Bcl-2 和Bax 蛋白的表达。结果显示：(1)与CON 组相比，I/R 组心肌缺血/ 再灌注后心肌凋亡细胞(TUNEL 染色)数量、凋亡率明显增加；(2)与I/R 组相比，POLY 组心肌缺血/ 再灌注后心肌凋亡细胞(TUNEL 染色)数量明显减少[(18.1±4.0)% vs (35.1±5.4)%,P<0.01]；(3)流式细胞术测定显示I/R 组心肌细胞凋亡率为(15.43±4.55)%，明显高于POLY 组心肌[(8.66±3.18)%] (P<0.01)；(4) I/R 组心肌Bax 蛋白表达明显高于POLY 组心肌(P<0.05)，POLY 组心肌Bcl-2 蛋白表达及Bcl-2/Bax 比值明显高于I/R 组心肌(P<0.05, P< 0.01)。以上结果表明，白藜芦醇甙可通过增加Bcl-2 蛋白表达，减少Bax 蛋白表达而减少I/R 诱导的大鼠心肌细胞凋亡。

关键词： 白藜芦醇甙; 细胞凋亡; 缺血/ 再灌注; 心脏; 大鼠

分类号：Q463

Polydatin attenuates ischemia/reperfusion-induced apoptosis in myocardium of the rat

ZHANG Li-Ping, MA Hui-Jie, BU Hui-Min, WANG Mei-Ling, LI Qian, QI Zhao, ZHANG Yi^*

Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China

Abstract

The aim of the present study was to investigate the effect of polydatin on apoptosis induced by ischemia/reperfusion (I/R) in rat myocardium and to explore the underlying mechanism. Adult male Sprague-Dawley (SD) rats were randomly divided into control, I/R and polydatin (50 μmol/L) groups. On the Langendorff apparatus, isolated rat heart was subjected to 30-min global ischemia followed by 60-min reperfusion. TUNEL labeling and flow cytometric techniques were used for the measurement of apoptosis and the expression of Bcl-2 and Bax protein in cardiomyocytes of rat. The results showed: (1) Compared with those in the control group, the number of TUNEL-positive cells and apoptosis rate were increased in I/R group; (2) Compared with that in the I/R group, the number of TUNEL-positive cells was significantly decreased in the polydatin group [(18.1±4.0)% vs (35.1±5.4)%, P<0.01]; (3) Apoptosis rate assayed by flow cytometry in I/R group was significantly higher than that in polydatin group [(15.43±4.55)% vs (8.66±3.18)%, P<0.01]; (4) Expression level of Bax protein was higher in I/R group than that in polydatin group (P<0.05), while the level of Bcl-2 protein and Bcl-2/Bax ratio were higher in polydatin group than those in I/R group (P<0.05, P<0.01), respectively. The results obtained suggest that polydatin exerts an inhibitory effect on I/R-induced apoptosis through increasing Bcl-2 protein expression and decreasing Bax protein expression in myocardium of the rat.

Key words: polydatin; apoptosis; ischemia/reperfusion; heart; rat

收稿日期：2009-04-23　　录用日期：2009-06-25

通讯作者：张翼　　E-mail: zhyhenry@hotmail.com

引用本文：

张利萍, 马会杰, 布会敏, 王妹玲, 李茜, 齐钊, 张翼. 白藜芦醇甙减轻缺血/ 再灌注诱发的大鼠心肌细胞凋亡[J]. 生理学报 2009; 61 (4): 367-372.

ZHANG Li-Ping, MA Hui-Jie, BU Hui-Min, WANG Mei-Ling, LI Qian, QI Zhao, ZHANG Yi. Polydatin attenuates ischemia/reperfusion-induced apoptosis in myocardium of the rat. Acta Physiol Sin 2009; 61 (4): 367-372 (in Chinese with English abstract).