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p38 MAPK通路介导胰高血糖素样肽-1对人脐静脉内皮细胞凋亡的抑制

徐华, 李海龙, 牛自勇, 李桂忠, 曹军, 姜怡邓

宁夏医科大学基础医学院病理生理学系，银川 750004；宁夏第三人民医院内科，银川 750021

摘要

本文旨在探讨胰高血糖素样肽-1 (glucagon-like peptide-1, GLP-1)对棕榈酸盐诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells, HUVECs)凋亡的影响及其机制。体外培养HUVECs，用棕榈酸盐诱导其凋亡，同时加入GLP-1干预。酶联接免疫吸附剂测定(enzyme-linked immunosorbnent assay, ELISA)法分别测定Caspase-3活性和DNA碎片含量，Western blot测定磷酸化细胞丝裂原活化蛋白激酶(phospho-p38 mitogen-activated protein kinase, p-p38 MAPK)表达。结果显示，与对照组相比，棕榈酸盐处理的HUVECs中Caspase-3活性和DNA碎片含量显著增多；而GLP-1可呈剂量依赖性降低棕榈酸盐所诱导升高的Caspase-3活性和DNA碎片。另外，GLP-1显著抑制由棕榈酸盐诱导的HUVECsp-p38 MAPK表达上调。以上结果提示，GLP-1可以抑制棕榈酸盐诱发的HUVECs脂性凋亡，其机制可能是对p-p38 MAPK表达的下调。

关键词： 胰高血糖素样肽-1; 人脐静脉内皮细胞; p38 MAPK; 凋亡

分类号：R363.2

[Involvement of p38 MAPK pathway in GLP-1-induced inhibition of apoptosis in human umbilical vein endothelial cells.] [Article in Chinese]

XU Hua, LI Hai-Long, NIU Zi-Yong, LI Gui-Zhong, CAO Jun, JIANG Yi-Deng

Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004； Department of Internal Medicine, Ningxia Third Hospital, Yinchuan 750021

Abstract

The aim of the present study was to investigate the effect of glucagon-like peptide-1 (GLP-1) on palmitate-induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the underlying mechanism. HUVECs were cultured in vitro, and then treated by palmitate to induce apoptosis. Meanwhile, GLP-1 was added to explore its effect. After 24 h of the treatments, Caspase-3 activity and DNA fragmentation were measured using ELISA kits. Phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) expression was detected by Western blot. The results showed that incubating HUVECs with 0.125 mmol/L GLP-1 increased Caspase-3 activity and DNA fragmentation. GLP-1 significantly inhibited palmitate-induced increases of Caspase-3 activity and DNA fragmentation in a concentration-dependent manner. Moreover, GLP-1 inhibited the up-regulation of p-p38 MAPK expression induced by palmitate in HUVECs. These results suggest GLP-1 protects HUVECs against lipo-apoptosis, and this effect may be mediated through inhibiting p38 MAPK pathway.

Key words: glucagon-like peptide-1; human umbilical vein endothelial cells; p38 MAPK; apoptosis

收稿日期：2012-02-28　　录用日期：2012-05-28

通讯作者：曹军,姜怡邓　　E-mail: jwcjyd@163.com, CAO Jun: Tel: +86-951-6980093

引用本文：

徐华, 李海龙, 牛自勇, 李桂忠, 曹军, 姜怡邓. p38 MAPK通路介导胰高血糖素样肽-1对人脐静脉内皮细胞凋亡的抑制[J]. 生理学报 2012; 64 (4): 444-448.

XU Hua, LI Hai-Long, NIU Zi-Yong, LI Gui-Zhong, CAO Jun, JIANG Yi-Deng. [Involvement of p38 MAPK pathway in GLP-1-induced inhibition of apoptosis in human umbilical vein endothelial cells.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (4): 444-448 (in Chinese with English abstract).