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NMDAR/PKC通路介导基底外侧杏仁核的长时程增强

陈爱琴, 陈晓春, 周瑞祥, 王玮

福建医科大学生理学及病理生理学系.福建,福州 350004；福建医科大学神经生物学研究中心.福建,福州 350004

摘要

该文在大鼠杏仁核脑片上刺激外囊(external capsule,EC),在基底外侧杏仁核(basolateral amygdala,BLA)记录场电位,观察能否在杏仁核诱导长时程增强(long-term potentiation,LTP),并探讨杏仁核LTP形成的可能机制。应用两串间隔10min的#thet#频率波刺激EC,可见BLA的场电位明显增强,持续时间在30min以上。EC--BLA的LTP表现为通路特异性,可被N--甲基--D--天冬氨酸受体(N--methyl--D--aspartate receptor,NMDAR)阻断剂D,L--2--氨基-5磷酸基戊酸(APV)阻断。在灌流液中加入蛋白激酶C(protein kinaseC,PKC)抑制剂chelerythrine chloride,对BLA的基础场电位和配对脉冲比率(pairedpulse ratio,PPR)没有影响;但在chelerythrine chloride存在的情况下,两串#thet#频率波刺激不能诱导出LTP;若于两串高频刺激10min后,在灌流液中加入chelerythrine chloride不能阻断BLA的LTP。上述结果表明,EC--BLA的LTP为NMDAR依赖性,PKC参与BLA的LTP诱导和早期维持。

关键词： 杏仁核; 场电位; 长时程增强; N-甲基-D-天冬氨酸受体; 蛋白激酶C

Involvement of protein kinase C in NMDAR--dependent long--term potentiation in rat amygdala

Chen Aiqin, Chen Xiaochun, Zhou Ruixiang, Wang Wei

Department of Physiology and Pathophysilology,Fujian Medical University.Fuzhou 350004,Fujian；China

Abstract

The mechanism of long-term potentiation(LTP) in basolateral amygdala(BLA) was explored using field potential recording in rat brain slice preparation.Field potentials(field excitatory post-synaptic potentials,fEPSPs) in BLA were evoked with sharpened steel bipolar stimulating electrodes placed in the external capsule(EC).Two #thet# burst stimulations(TBS,interval=10 min) induced LTP in BLA.TBS-induced synaptic potentiation lasted for more than 30 min after the second TBS.LTP in BLA was input-specific and was blocked by N-methyl-D-aspartate receptor(NMDAR) antagonist 2-amino-5-phosphonovaleric acid(APV).The effect of protein kinase C(PKC) on LTP was then determined using PKC inhibitor chelerythrine chloride.Bath application of chelerythringe chloride had no effect on basic field potentials and paired-pulse ratio(PPR).However,in the presence of chelerythrine chloride,two TBS failed to induce LTP.In contrast,bath application of chelerythrine chloride 10 min after the second TBS did not affect the maintenance of LTP in BLA.These results indicate that LTP is NMDAR-dependent and PKC is involved in the induction and early maintenance of LTP in BLA.

Key words: Amygdala;field potentials;lont-term potentiation;N-methyl-D-aspartate receptor;Protein kinase C

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引用本文：

陈爱琴, 陈晓春, 周瑞祥, 王玮. NMDAR/PKC通路介导基底外侧杏仁核的长时程增强[J]. 生理学报 2008; 60 (6): 737-742.

Chen Aiqin, Chen Xiaochun, Zhou Ruixiang, Wang Wei. Involvement of protein kinase C in NMDAR--dependent long--term potentiation in rat amygdala. Acta Physiol Sin 2008; 60 (6): 737-742 (in Chinese with English abstract).