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肺内调节肽对人支气管上皮细胞HLA--DR、CD80、CD86表达的影响

彭丽花, 秦晓群, 谭宇蓉, 向阳, 刘惠君

湖南永州职业技术学院生理学教研室.湖南,永州 425100；中南大学湘雅医学院生理学系.湖南,长沙 410078

摘要

为了探讨肺内调节肽对人支气管上皮细胞(human bronchial epithelial cells,HBECs)人类白细胞抗原DR(human leukocyte antigen DR,HLA--DR)、CD80和CD86表达的影响,采用免疫细胞化学技术和流式细胞术检测HBECs在非应激和臭氧应激两种状态下HLA--DR、CD80、CD86的表达。结果显示,HBECs表达HLA--DR,臭氧应激状态下HBECsHLA---DR表达降低({sl P}<0.05);VIP、P3513和CGRP使非应激和臭氧应激两种状态下的HBECsHLA-DR表达增高(均{sl P}<0.05)。HBECs表达协同刺激分子CD80,臭氧应激状态下CD80表达降低({sl P}<0.05),VIP对非应激的HBECs的CD80表达无影响,使臭氧应激状态下CD80表达增高({sl P}<0.05);CGRP使非应激状态下的HBECs的CD80表达降低({sl P}<0.05),使臭氧应激状态下CD80表达增高({sl P}<0.05);P3513使非应激状态下CD80表达增高({sl P}<0.05),可使臭氧应激状态下CD80表达降低({sl P}<0.05)。在HBECs没有检测到CD86表达,臭氧攻击也不能刺激其表达。上述结果提示,HBECs具备成为抗原递呈细胞的必要条件,肺内调节肽可通过调节HLA--DR和协同刺激分子的表达调节HBECs的抗原递呈作用。

关键词： 人支气管上皮细胞; 肺内调节肽; 抗原递呈分子; 臭氧

Influence of intrapulmonary regulatory peptides on the expressions of HLA--DR,CD80 and CD86 in human bronchial epithelial cells

Peng Lihua, Qin Xiaoqun, Tan Yurong, Xiang Yang, Liu Huijun

Department of Physiology,Hunan Yongzhou Vocational Technical College.Yongzhou 425100,Hunan；China

Abstract

Antigen presenting is the initial step of the immune responses.In order to verify that human bronchial epithelial cells(HBECs) can express antigen presentation molecules,which can be modulated by intrapulmonary regulatory peptides,the present study was designed to examine the expressions of human leukocyte antigen DR(HLA-DR),CD80 and CD86 in resting or ozone-stressed HBECs by using immunocytochemistry and flow cytometry analysis.The results showed that HBECs expressed HLA-DR,CD80 and the expressions of HLA-DR and CD80 molecules were down-regulated under ozone stress.While VIP,P3513 and CGRP upregulated the expression of HLA-DR in resting or ozone-stressed HBECs,they had different effects on CD80 expression.VIP did not influence the expression of CD80 under resting state,but increased the expression of CD80 under ozone stress.CGRP decreased CD80 expression in resting HBECs,but increased CD80 expression in ozone-stressed HBECs.P3513 increased CD80 expression in resting HBECs,but decreased CD80 expression in ozone-stressed HBECs.The expression of CD86 was absent in resting or ozone-stressed HBECs.The results obtained demonstrate that HBECs have the capability to act as antigen presenting cells and the expression of HLA-DR and costimulatory molecules can be modulated by intrapulmonary regulatory peptides.

Key words: human bronchial epithelial cells;intrapulmonary regulatory peptides;antigen presentation molecules;ozone

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引用本文：

彭丽花, 秦晓群, 谭宇蓉, 向阳, 刘惠君. 肺内调节肽对人支气管上皮细胞HLA--DR、CD80、CD86表达的影响[J]. 生理学报 2008; 60 (6): 723-729.

Peng Lihua, Qin Xiaoqun, Tan Yurong, Xiang Yang, Liu Huijun. Influence of intrapulmonary regulatory peptides on the expressions of HLA--DR,CD80 and CD86 in human bronchial epithelial cells. Acta Physiol Sin 2008; 60 (6): 723-729 (in Chinese with English abstract).