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不同激动剂引起大鼠肺动脉主干与三级分支收缩反应的比较

黄秋虹, 穆云萍, 颜芙蓉, 朱洁玲, 刘晓如, 林默君

福建医科大学基础医学院生理学与病理生理学系；福建医科大学心血管科学研究室，福州 350108；泉州医学高等专科学校，泉州 362000

摘要

为观察苯肾上腺素(phenylephrine, Phen)、内皮素-1 (endothelin-1, ET-1)和KCl引起大鼠肺动脉主干与三级分支间收缩反应的差异，本文分别用传统的大血管张力检测技术和DMT微血管张力检测技术，记录KCl、Phen和ET-1引起的去内皮肺动脉主干和三级分支血管张力变化。结果显示：Phen可引起肺动脉主干显著收缩，但是不引起肺动脉三级分支的明显收缩；与肺动脉主干相比，ET-1导致肺动脉三级分支的收缩反应减弱，反应的最大值和亲和力指数(pD2)值均降低；与肺动脉主干相比，KCl引起肺动脉三级分支的收缩反应增强。上述结果说明，肺动脉主干和肺动脉三级分支的血管反应性存在差异，进行肺小动脉血管功能研究具有重要意义。本实验可为进一步研究肺动脉小分支的血管功能及其在肺高压中的改变提供重要实验依据。

关键词： 肺动脉主干; 肺动脉三级分支 ; KCl ; 内皮素-1 ; 苯肾上腺素

分类号：R3

Comparison of agonists-induced contraction between main and the third-order branches of pulmonary arteries in rats

HUANG Qiu-Hong, MU Yun-Ping, YAN Fu-Rong, ZHU Jie-Ling, LIU Xiao-Ru, LIN Mo-Jun

Department of Physiology and Pathophysiology； Lab of Cardiovascular Sciences, Fujian Medical University, Fuzhou 350108, China； Quanzhou Medical College, Quanzhou 362000, China

Abstract

This study was designed to observe the differences between main pulmonary arteries and the third-order branches of pulmonary arteries in the contractile response to phenylephrine (Phen), endothelin-1 (ET-1) and potassium chloride (KCl). The vascular tension changes of main and the third-order branches of pulmonary arteries induced by KCl, ET-1 and Phen were recorded by traditional vascular tone detection methods and microvascular ring technique, respectively. The results showed that Phen could cause a significant contraction in main pulmonary arteries, but did not induce apparent contraction in the third-order branches of pulmonary arteries. Compared with main pulmonary arteries, ET-1 contracted the third-order branches of pulmonary arteries with reduced maximal response value and PD2 value. In comparison with the main pulmonary arteries, contraction caused by KCl was enhanced in the third-order branches of pulmonary arteries. The results suggest that the vascular reactivity of main and the third-order branches of pulmonary arteries is different and it is important to study the vascular function of small branches of pulmonary arteries. This study could provide an important experimental basis for the further study on vascular function of small branches of pulmonary arteries and the functional changes in pulmonary hypertension.

Key words: main pulmonary arteries; the third-order branches of pulmonary arteries ; potassium chloride ; endothelin-1 ; phenylephrine

收稿日期：2016-06-17　　录用日期：2016-08-29

通讯作者：林默君　　E-mail: mjlin@mail.fjmu.edu.cn

引用本文：

黄秋虹, 穆云萍, 颜芙蓉, 朱洁玲, 刘晓如, 林默君. 不同激动剂引起大鼠肺动脉主干与三级分支收缩反应的比较[J]. 生理学报 2017; 69 (1): 1-10.

HUANG Qiu-Hong, MU Yun-Ping, YAN Fu-Rong, ZHU Jie-Ling, LIU Xiao-Ru, LIN Mo-Jun. Comparison of agonists-induced contraction between main and the third-order branches of pulmonary arteries in rats. Acta Physiol Sin 2017; 69 (1): 1-10 (in Chinese with English abstract).