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阿片受体在急性应激引起的识别记忆变化中的作用

刘迎, 吴宇蔚, 钱召强, 闫彩芳, 范卡敏, 胥瑾慧, 李晓, 刘志强^*

陕西师范大学现代教学技术教育部重点实验室，西安 710062

摘要

多项研究结果表明急性应激会损伤动物的学习记忆，但对获取、巩固及提取阶段的影响不尽相同。同时，有关人类和动物的实验表明内源性阿片系统与应激也密切相关，应激后脑内内源性阿片类物质的表达和释放量会上升。然而，内源性阿片系统是否参与应激后学习记忆功能的损伤尚未明确。本研究旨在通过新物体识别实验三天范式来探讨强迫游泳应激对C57小鼠识别记忆的影响以及阿片受体在其中的作用。结果显示，急性强迫游泳应激损伤小鼠识别记忆的提取，而对其识别记忆的获取及巩固影响不明显；腹腔注射阿片受体拮抗剂纳洛酮本身对小鼠的物体识别记忆没有影响，但可以反转应激对于识别记忆提取的损伤。采用实时定量PCR技术对全脑、海马和前额叶μ阿片受体转录水平相对表达量进行观察，结果显示，强迫游泳应激后全脑和海马μ阿片受体的mRNA相对含量有所下降，而预注射纳洛酮可以反转这种变化。上述结果提示急性应激可能通过阿片受体损伤识别记忆的提取。

关键词： 阿片受体; 急性应激; 识别记忆; 荧光定量PCR

分类号：Q426

[Effect of opioid receptors on acute stress-induced changes in recognition memory.] [Article in Chinese]

LIU Ying, WU Yu-Wei, QIAN Zhao-Qiang, YAN Cai-Fang, FAN Ka-Min, XU Jin-Hui, LI Xiao, Liu Zhi-Qiang^*

Key Laboratory of Modern Teaching Technology, Shaanxi Normal University, Xi’an 710062, China

Abstract

Although ample evidence has shown that acute stress impairs memory, the influences of acute stress on different phases of memory, such as acquisition, consolidation and retrieval, are different. Experimental data from both human and animals support that endogenous opioid system plays a role in stress, as endogenous opioid release is increased and opioid receptors are activated during stress experience. On the other hand, endogenous opioid system mediates learning and memory. The aim of the present study was to investigate the effect of acute forced swimming stress on recognition memory of C57 mice and the role of opioid receptors in this process by using a three-day pattern of new object recognition task. The results showed that 15-min acute forced swimming damaged the retrieval of recognition memory, but had no effect on acquisition and consolidation of recognition memory. No significant change of object recognition memory was found in mice that were given naloxone, an opioid receptor antagonist, by intraperitoneal injection. But intraperitoneal injection of naloxone before forced swimming stress could inhibit the impairment of recognition memory retrieval caused by forced swimming stress. The results of real-time PCR showed that acute forced swimming decreased the μ opioid receptor mRNA levels in whole brain and hippocampus, while the injection of naloxone before stress could reverse this change. These results suggest that acute stress may impair recognition memory retrieval via opioid receptors.

Key words: Opioid receptor; acute stress; recognition memory; naloxone

收稿日期：2016-04-27　　录用日期：2016-08-23

通讯作者：刘志强　　E-mail: zqliu1969@163.com

引用本文：

刘迎, 吴宇蔚, 钱召强, 闫彩芳, 范卡敏, 胥瑾慧, 李晓, 刘志强. 阿片受体在急性应激引起的识别记忆变化中的作用[J]. 生理学报 2016; 68 (6): 757-766.

LIU Ying, WU Yu-Wei, QIAN Zhao-Qiang, YAN Cai-Fang, FAN Ka-Min, XU Jin-Hui, LI Xiao, Liu Zhi-Qiang. [Effect of opioid receptors on acute stress-induced changes in recognition memory.] [Article in Chinese]. Acta Physiol Sin 2016; 68 (6): 757-766 (in Chinese with English abstract).