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斑马鱼依托咪酯麻醉模型的建立

杜文杰, 杜久林, 喻田^*

1遵义医学院，贵州省麻醉与器官保护重点实验室，遵义 563000；2中国科学院上海生命科学研究院，神经科学研究所，上海 200031

摘要

全身麻醉药广泛应用于临床，但其引起全身麻醉状态的神经机制至今仍不清楚。脊椎动物斑马鱼具有保守而简单的脑结构，近几年来已应用于神经机理基础性的研究。在本工作中，我们从行为和电生理水平上，首次建立了斑马鱼麻醉模型。在细胞外液中施加静脉麻醉药依托咪酯(etomidate)，可以浓度依赖性地抑制斑马鱼的运动。在体细胞外电生理记录显示，依托咪酯可有效阻断斑马鱼脊髓运动神经元的电活动。运用在体局部场电位和全细胞记录技术，进一步发现依托咪酯可显著抑制大脑群体神经元的活动，并阻断视觉信号的传递。本工作表明，斑马鱼可以作为一种合适的动物模型，应用于全身麻醉药神经机制的研究。

关键词： 斑马鱼; 全身麻醉; 依托咪酯; 在体电生理记录; 行为

Establishment of an anesthesia model induced by etomidate in larval zebrafish

DU Wen-Jie, DU Jiu-Lin, YU Tian^*

1Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical College, Zunyi 563000, China； 2Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

Abstract

Despite the wide application of general anesthetic drugs in clinic, it is still unclear how these drugs induce the state of general anesthesia. Larval zebrafish has emerged as an ideal model for dissecting the mechanism of neural systems due to the conserved and simple brain structure. In the present study, we established an anesthesia model from behavioral to electrophysiological levels using larval zebrafish for the first time. Bath application of etomidate, as a kind of intravenous anesthetic drugs, suppressed the spontaneous locomotion of zebrafish in a concentration-dependent manner. Consistently, in vivo fictive motor patterns of spinal motoneurons recorded extracellularly were significantly inhibited as well. Furthermore, using in vivo extracellular recording and whole-cell recording, we found that etomidate application suppressed local field potentials (LFP) of the brain and blocked visually evoked responses of optic tectal neurons. The study indicates that larval zebrafish can serve as an ideal vertebrate animal model for studying neural mechanisms underlying general anesthesia.

Key words: zebrafish; general anesthesia; etomidate; in vivo electrophysiological recording; behavior

收稿日期：2016-01-11　　录用日期：2016-02-02

通讯作者：喻田　　E-mail: zyyutian@126.com

引用本文：

杜文杰, 杜久林, 喻田. 斑马鱼依托咪酯麻醉模型的建立[J]. 生理学报 2016; 68 (3): 301-305.

DU Wen-Jie, DU Jiu-Lin, YU Tian. Establishment of an anesthesia model induced by etomidate in larval zebrafish. Acta Physiol Sin 2016; 68 (3): 301-305 (in Chinese with English abstract).