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屋尘螨和卵蛋白对小鼠气道上皮粘附分子整合素β4和细胞间粘附分子-1表达的影响

曾丹, 谭眉灵, 向阳, 秦晓群, 朱黎明, 戴爱国

湖南省老年医院呼吸系统疾病研究所，长沙 410016；中南大学基础医学院生理学系，长沙 410013

摘要

为了探明不同变应原与气道粘附分子之间的关系，本研究采用屋尘螨(house dust mite, HDM)和卵蛋白(ovalbumin, OVA)分别致敏BABL/c小鼠，采用Buxco小动物肺功能分析系统观察气道的反应性，计数外周血白细胞数目，肺部HE染色观察气道的病理改变，利用免疫组织化学技术检测气道上皮粘附分子整合素β4 (integrin β4, ITGB4)和细胞间粘附分子-1 (intercellular adhesion molecule-1, ICAM-1)的表达。结果显示，与对照组比较，HDM组和OVA组小鼠外周血白细胞数目明显增加，肺部炎症细胞浸润，气道上皮ITGB4表达下调，而ICAM-1表达上调。然而，OVA组小鼠气道上皮ICAM-1的表达上调较HDM组更为明显，炎症细胞的浸润也更严重。采用siRNA沉默16HBE人支气管上皮细胞株ITGB4的表达后，ICAM-1的表达上调。以上结果均提示，变应原能够通过改变气道粘附分子谱来影响肺内炎症反应。

关键词： 屋尘螨; 卵蛋白; 粘附分子; 整合素β4; 细胞间粘附分子-1

分类号：R56

[Allergens-induced sensitization alters airway epithelial adhesion molecules expression in mice.] [Article in Chinese]

ZENG Dan, TAN Mei-Ling, XIANG Yang, QIN XIAO-QUN, ZHU Li-Ming, DAI Ai-Guo

Department of Respiratory Medicine, Geriatric Hospital of Hunan Province, Changsha 410016, China； Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410013, China

Abstract

To explore the relationship between the epithelial adhesion molecules and immune responses of airway epithelium, we observed the expression of integrin β4 and intercellular adhesion molecule-1 (ICAM-1) in the mice airway epithelium after sensitization with allergens. BALB/c mice were sensitized with intraperitoneal injection of ovalbumin (OVA) or house dust mite (HDM) and then developed airway hyper-responsiveness as determined by barometric whole-body plethysmography. Both OVA and HDM sensitization led to increases of the number of peripheral leukocytes as well as inflammatory cells infiltration in lungs. OVA sensitized mice showed more severe inflammatory cells infiltration than HDM sensitized mice. Immunohistochemistry analysis of mice lung tissues revealed that sensitization with both allergens also led to a decrease of integrin β4 expression and an increase of ICAM-1 expression in airway epithelia. OVA sensitized mice showed a more significant increase of ICAM-1 expression compared with HDM sensitized mice. siRNA mediated silencing of integrin β4 gene in 16HBE cells resulted in an up-regulation of ICAM-1 expression. Our results indicate a possible role of airway epithelial adhesion molecules in allergen-induced airway immune responses.

Key words: house dust mite; ovalbumin; adhesion molecules; integrin β4; intercellular adhesion molecule-1

收稿日期：2015-06-17　　录用日期：2015-06-17

通讯作者：秦晓群　　E-mail: qinxiaoqun@csu.edu.cn

引用本文：

曾丹, 谭眉灵, 向阳, 秦晓群, 朱黎明, 戴爱国. 屋尘螨和卵蛋白对小鼠气道上皮粘附分子整合素β4和细胞间粘附分子-1表达的影响[J]. 生理学报 2015; 67 (6): 596-602.

ZENG Dan, TAN Mei-Ling, XIANG Yang, QIN XIAO-QUN, ZHU Li-Ming, DAI Ai-Guo. [Allergens-induced sensitization alters airway epithelial adhesion molecules expression in mice.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (6): 596-602 (in Chinese with English abstract).