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Flor-Essence营养补剂对运动大鼠IL-6、IL-12、肿瘤坏死因子-α和自然杀伤细胞的影响

林华, 贺业恒, 徐瑞, 邹伟

辽宁师范大学体育学院，大连 116029；大连市体育科学研究所，大连 116031；辽宁师范大学生命科学学院，大连 116029

摘要

本文旨在探讨运动和Flor-Essence营养补剂干预对大鼠免疫功能的影响。将8周龄Sprague-Dawley (SD)大鼠随机分为6组：对照组、生理盐水 + 训练组、低剂量Flor-Essence + 训练组、低剂量Flor-Essence组、高剂量Flor-Essence + 训练组和高剂量Flor-Essence组。生理盐水 + 训练组、低剂量Flor-Essence + 训练组和高剂量Flor-Essence + 训练组大鼠每日在水箱中进行游泳训练，时间为35 min/天，每周训练6天，持续4周。低剂量Flor-Essence + 训练组、低剂量Flor-Essence组、高剂量Flor- Essence + 训练组和高剂量Flor-Essence组每日训练前灌胃2.5 (低剂量)或5 mg/mL (高剂量) Flor-Essence补剂，生理盐水 + 训练组灌胃同体积生理盐水。在第4周最后一天对所有大鼠进行一次性力竭运动，力竭后即刻处死，取动脉血用试剂盒测定血清白细胞介素-6 (IL-6)、白细胞介素-12 (IL-12)及肿瘤坏死因子-α (TNF-α)含量，取脾制备单细胞悬液测定自然杀伤细胞(NK细胞)活性。结果显示，生理盐水 + 训练组大鼠血清IL-6含量低于对照组。与对照组相比较，低剂量和高剂量Flor-Essence组血清IL-6、IL-12和TNF-α含量降低，力竭运动时间增加。5 mg/mL Flor-Essence对于大鼠血清IL-6、TNF-α含量和力竭运动时间的改善作用要强于2.5 mg/mL Flor-Essence。与生理盐水 + 训练组相比，低剂量和高剂量Flor-Essence + 训练组血清IL-6、TNF-α含量均降低，力竭运动时间增加；只有高剂量Flor-Essence + 训练组血清IL-12含量显著降低，NK细胞活性显著增加。以上结果提示，Flor-Essence结合运动可以改善大鼠免疫功能和运动机能，且5 mg/mL浓度的作用效果要优于2.5 mg/mL。

关键词： Flor-Essence补剂; 运动大鼠; 力竭运动; 免疫指标

分类号：Q4

[Effect of Flor-Essence on serum levels of IL-6, IL-12, TNF-α and NK cells in exercise rats.] [Article in Chinese]

LIN Hua, HE Ye-Heng, XU Rui, ZOU Wei

Physical Education College of Liaoning Normal University, Dalian 116029, China； Sports Science Research Institute of Dalian, Dalian 116031, China； College of Life Science of Liaoning Normal University, Dalian 116029, China

Abstract

he present study was aimed to investigate the effects of exercise and nutrition intervention on rat immune function. Flor-Essence is a kind of health food produced by FLORA company in Canada and certified by Quality Assurance International (QAI). Its main components are burdock root, cress leaves of grass, kelp, Turkish rhubarb root, et al. Flor-Essence has been shown to activate the body detoxification path, improve the physical environment, and inhibit cancer cell growth and proliferation. Sixty male Sprague-Dawley (SD) rats were randomly divided into six groups: control, NS + training, low-dose Flor-Essence + training, low-dose Flor-Essence, high-dose Flor-Essence + training, high-dose Flor-Essence groups. The rats in NS + training, low-dose Flor-Essence + training, high-dose Flor-Essence + training groups swam 35 min per day in the water tank for 6 days a week. One hour before exercise, the rats were given low- (2.5 mg/mL) or high-dose (5 mg/mL) Flor-Essence daily by intragastric administration, and the rats in NS + training group were given equivalent volume of NS. On the last day of four training weeks, all rats took part in a bout of exhaustive exercise, and then were sacrificed immediately. Arterial blood serum samples were taken for the assays of IL-6, IL-12 and TNF-α contents, spleens for natural killer (NK) cells activity. The results showed that serum IL-6 content in NS + training group was decreased compared with that in control group. Low- and high-dose Flor-Essence groups showed decreased IL-6, IL-12 and TNF-α serum contents, as well as longer exhaustive time, compared with control group. The improving effects of high-dose Flor- Essence on IL-6, TNF-α and exhaustive time were greater than those of low dose. Compared with NS + training, low- and high-dose Flor-Essence + training reduced serum contents of IL-6 and TNF-α, and prolonged exhaustive time; only high-dose Flor-Essence + training decreased serum IL-12 content and enhanced NK cells activity. These results suggest Flor-Essence in combination with exercise can improve rat immune function and sports performance, and the effect of high-dose Flor-Essence is better than that of low dose.

Key words: Flor-Essence; exercise rats; exhaustive exercise; immune indexes

收稿日期：2015-04-20　　录用日期：2015-09-16

通讯作者：林华,贺业恒　　E-mail: ,

引用本文：

林华, 贺业恒, 徐瑞, 邹伟. Flor-Essence营养补剂对运动大鼠IL-6、IL-12、肿瘤坏死因子-α和自然杀伤细胞的影响[J]. 生理学报 2015; 67 (6): 618-622.

LIN Hua, HE Ye-Heng, XU Rui, ZOU Wei. [Effect of Flor-Essence on serum levels of IL-6, IL-12, TNF-α and NK cells in exercise rats.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (6): 618-622 (in Chinese with English abstract).