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第四脑室注射orexin-A及OX1R拮抗剂对大鼠摄食及自由活动的影响

彭晓燕, 郭菲菲, 孙向荣, 公衍玲, 徐珞

青岛大学医学院病理生理教研室，青岛 266021；青岛科技大学化工学院病理生理教研室，青岛 266021

摘要

本文旨在探讨第四脑室注射orexin-A及orexin 1型受体(orexin-1 receptor, OX1R)拮抗剂SB334867对肥胖和正常大鼠摄食和自由活动的影响。采用高脂饲料诱导建立肥胖大鼠模型，分别在肥胖和正常大鼠第四脑室注射不同剂量orexin-A或SB334867，观察光照和黑暗环境下两种大鼠0~4 h摄食量及活动量的变化。结果显示，第四脑室注射不同剂量orexin-A，光照条件下，正常和肥胖大鼠0~4 h摄食量和活动量均较生理盐水对照组明显增加，呈剂量依赖关系(P < 0.05~0.01)；且肥胖大鼠摄食量和活动量显著高于正常大鼠；黑暗条件下，不同剂量的orexin-A对正常和肥胖大鼠摄食量和活动量均没有明显改变(P > 0.05)。第四脑室注射不同剂量SB334867，光照条件下，正常和肥胖大鼠0~2 h，2~4 h摄食量和活动量均较生理盐水对照组明显减少(P < 0.05)；且肥胖大鼠摄食量和活动量均显著高于正常大鼠；黑暗条件下，正常和肥胖大鼠摄食量和活动量均没有明显改变(P > 0.05)。以上结果提示，第四脑室周核团可能是orexin-A及OX1R作用靶点之一；光照条件对orexin-A和OX1R生理功能的发挥可能具有重要影响。

关键词： orexin-A; SB334867; 摄食; 自由活动; 肥胖; 大鼠

Effect of orexin-A and orexin-1 receptor antagonist injected into the fourth ventricle of rats on food-intake and spontaneous physical activity

PENG Xiao-Yan, GUO Fei-Fei, SUN Xiang-Rong, GONG Yan-Ling, XU Luo

Department of Pathophysiology, Qingdao University Medical College, Qingdao 266021, China； Department of Pathophysiology, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266021, China

Abstract

The present study was aimed to investigate the effects of orexin-A and orexin-1 receptor (OX1R) antagonist injected into the fourth ventricle of rats on food-intake and spontaneous physical activity (SPA). Obese rat model was induced by high fat diet. Different doses of orexin-A or SB334867, an OX1R antagonist, were injected into the fourth ventricle of obese and normal rats respectively. SPA and food intake were monitored for 4 h after injection in both light and dark environment. In the light measurement cycle, different doses of orexin-A significantly stimulated feeding and SPA in all injected rats, and the animals’ responses showed a dose-dependent manner (P < 0.05–0.01), and compared with those of normal rats, the orexin-A induced food intake and SPA were more pronounced in obese rats. In the dark measurement cycle, different doses of orexin-A had no obvious effect on food intake and SPA in both normal and obese rats (P > 0.05). In the light cycle, different doses of SB334867 significantly decreased food intake and SPA in all rats during 0–2 h and 2–4 h after injection (P < 0.05), but the food intake and SPA in obese rats were significantly greater than those of normal rats. In the dark cycle, different doses of SB334867 showed no obvious effect on food intake and SPA of normal and obese rats (P > 0.05). These results suggest that fourth cerebral ventricle nuclei may be one target for orexin-A and light condition may play an important role in orexin-A and OX1R physiological functional processes.

Key words: orexin-A; SB334867; food intake; spontaneous physical activity; obesity; rats

收稿日期：2015-04-20　　录用日期：2015-05-25

通讯作者：徐珞　　E-mail: fluocn@gmail.com

引用本文：

彭晓燕, 郭菲菲, 孙向荣, 公衍玲, 徐珞. 第四脑室注射orexin-A及OX1R拮抗剂对大鼠摄食及自由活动的影响[J]. 生理学报 2015; 67 (4): 379-385.

PENG Xiao-Yan, GUO Fei-Fei, SUN Xiang-Rong, GONG Yan-Ling, XU Luo. Effect of orexin-A and orexin-1 receptor antagonist injected into the fourth ventricle of rats on food-intake and spontaneous physical activity. Acta Physiol Sin 2015; 67 (4): 379-385 (in Chinese with English abstract).