过刊浏览

生长激素释放肽-6对小鼠下丘脑摄食相关核团c-fos表达时间依从性影响

俞燕娟, 董静, 蒋正尧, Inge Depoortere

青岛大学医学院1特种医学专业；生理学教研室，青岛 266071；鲁汶大学胃肠激素研究中心，鲁汶，比利时

摘要

本研究旨在探讨外周注射ghrelin受体激动剂生长激素释放肽-6 (growth hormone releasing peptide-6, GHRP-6)对NMRI小鼠摄食的影响以及摄食相关核团(弓状核、视上核和室旁核)的激活情况及其有效作用时间。腹腔注射GHRP-6 1，3，6 h后观察小鼠累计摄食量，同时用免疫组织化学方法检测GHRP-6对自由饮食小鼠和禁食小鼠下丘脑摄食相关核团c-fos表达影响，并观察GHRP-6作用的时间依从性。结果显示，腹腔注射了GHRP-6的小鼠摄食量明显大于生理盐水注射鼠，且在注射后3 h时观察到的摄食量的增加尤为显著，但注射后6 h内总的累计摄食量无显著变化；同时，GHRP-6能够在不依赖于摄食的情况下促进弓状核和室旁核中c-fos的表达，且c-fos的表达在注射后1 h时达到峰值，随后逐渐下降。以上结果提示，外源性注入GHRP-6可显著增加动物在给药后1、3 h的累积摄食量，该作用至少部分是通过上调弓状核和室旁核中的c-fos蛋白表达起作用的，而且具有时间依从性。本研究结果可为临床ghrelin受体激动剂的使用间隔提供理论依据。

关键词： 生长激素释放肽-6; 摄食 ; 下丘脑 ; c-Fos

分类号：R335

Time course study of growth hormone releasing peptide-6-induced c-fos expression in neurons of feeding-related nuclei of hypothalamus

YU Yan-Juan, DONG Jing, JIANG Zheng-Yao, Inge Depoortere

Department of Special Medicine； Department of Physiology, Qingdao Medical College of Qingdao University, Qingdao 266071, China； Center for Gastroenterological Research, KU Leuven, Leuven, Belgium

Abstract

The present study was aimed to explore the effects of intraperitoneal injection of growth hormone releasing peptide-6 (GHRP-6), a ghrelin receptor agonist, on food intake and neuronal activity of feeding-related nuclei in the hypothalamus of NMRI mice. Accumulated amount of food intake was measured, and total number of c-fos immunoreactive neurons in arcuate nucleus (ARC), paraventricular nucleus (PVN) and supraoptic nucleus (SON) was counted by immunohistochemistry at 1, 3 and 6 h after the GHRP-6 injection. The results showed that GHRP-6 significantly increased the amount of food intake with a peak at 3 h after the GHRP-6 injection. Meanwhile, GHRP-6 could promote c-fos expression in the ARC and PVN independent of food intake, and the total number of c-fos immunoreactive neurons was peaked at 1 h after injection and then decreased gradually. These results suggest that GHRP-6 may increase food intake in time-dependent manner, which is associated with up-regulations of c-fos protein expression in the ARC and PVN.

Key words: GHRP-6; food intake ; hypothalamus ; c-Fos

收稿日期：2015-09-13　　录用日期：2015-11-17

通讯作者：董静　　E-mail: dongjing6@hotmail.com

引用本文：

俞燕娟, 董静, 蒋正尧, Inge Depoortere. 生长激素释放肽-6对小鼠下丘脑摄食相关核团c-fos表达时间依从性影响[J]. 生理学报 2016; 68 (1): 19-26.

YU Yan-Juan, DONG Jing, JIANG Zheng-Yao, Inge Depoortere. Time course study of growth hormone releasing peptide-6-induced c-fos expression in neurons of feeding-related nuclei of hypothalamus. Acta Physiol Sin 2016; 68 (1): 19-26 (in Chinese with English abstract).