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褪黑素的抗胃癌效应及其引起的Bcl-2、Bax、p21和p53的表达变化

徐丽, 金清东, 宫喜, 刘卉, 周瑞祥

莆田学院基础医学院生理学教研室，莆田 351100；福建医科大学人体解剖学与组织胚胎学系，神经生物学研究中心，福州 350004

摘要

为了探讨褪黑素体内抗小鼠胃癌作用及其机制，本研究应用小鼠胃癌(murine foregastric carcinoma, MFC)细胞建立荷胃癌小鼠模型，不同浓度褪黑素干预后，运用实时荧光定量RT-PCR、免疫印迹等方法检测Bcl-2，Bax，p21，p53的表达变化。结果显示：(1)褪黑素能够缩小荷胃癌小鼠的肿瘤体积，减轻肿瘤重量，达到抗胃癌作用；(2)褪黑素在抑制胃癌生长过程中下调肿瘤组织Bcl-2表达，上调Bax、p21和p53表达。以上结果表明，褪黑素可通过促进胃癌细胞凋亡从而实现体内抑制胃癌生长作用。

关键词： 胃癌; 褪黑素; 小鼠胃癌细胞; Bcl-2; Bax; p21; p53

分类号：R735.2

[Anti-gastric cancer effect of melatonin and Bcl-2, Bax, p21 and p53 expression changes.] [Article in Chinese]

XU Li, JIN Qing-Dong, GONG Xi, LIU Hui, ZHOU Rui-Xiang

Department of Physiology, Basic Medical College of Putian University, Putian 351100, China； Department of Human Anatomy and Histology and Embryology, Neurobiology Research Center, Fujian Medical University, Fuzhou 350004, China

Abstract

In order to investigate the role of melatonin in inhibiting the proliferation of murine gastric cancer and the underlying molecular mechanism, we performed an in vivo study by inoculating murine foregastric carcinoma (MFC) cells in mice, and then tumor-bearing mice were treated with different concentrations of melatonin (i.p.). The changes of Bcl-2, Bax, p21 and p53 expressions in tumor tissue were detected by using real-time fluorescence quantitative RT-PCR and Western blot. We found that: (1) melatonin resulted in reductions of tumor's volume and weight in the gastric cancer-bearing mice and thus showed anti-cancer effect; (2) melatonin reduced Bcl-2 expression, but increased the expression of Bax, p53 and p21 in tumor tissue. Our results suggest that melatonin could inhibit the growth of tumors in gastric cancer-bearing mice through accelerating the apoptosis of tumor cells.

Key words: gastric cancer; melatonin; murine foregastric carcinoma; Bcl-2; Bax; p21; p53

收稿日期：2014-05-27　　录用日期：2014-07-17

通讯作者：周瑞祥　　E-mail: zhourx@mail.fjmu.edu.cn

引用本文：

徐丽, 金清东, 宫喜, 刘卉, 周瑞祥. 褪黑素的抗胃癌效应及其引起的Bcl-2、Bax、p21和p53的表达变化[J]. 生理学报 2014; 66 (6): 723-729.

XU Li, JIN Qing-Dong, GONG Xi, LIU Hui, ZHOU Rui-Xiang. [Anti-gastric cancer effect of melatonin and Bcl-2, Bax, p21 and p53 expression changes.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (6): 723-729 (in Chinese with English abstract).