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5,7-双羟色胺侧脑室注射对大鼠内侧前额叶皮层神经元兴奋性的影响

范玲玲^*, 胡志红, 周玉东, 李倩倩, 贾焱如, 王金峰, 赵春伟

河南科技大学医学院，洛阳 471003

摘要

本研究采用在体细胞外记录的方法，探讨了双侧侧脑室注射5,7-双羟色胺(5,7-dihydroxytryptamine, 5,7-DHT)损毁大鼠脑内5-羟色胺(5-hydroxytryptamine, 5-HT)神经元后，内侧前额叶皮层(medial prefrontal cortex, mPFC)锥体和中间神经元兴奋性的变化。实验结果显示：5,7-DHT注射到双侧侧脑室后第2周，mPFC和中缝背核内的5-HT水平较正常组显著下降。mPFC的锥体神经元的放电频率与正常组相比明显升高，爆发式放电显著增多，而中间神经元的平均放电频率显著降低，放电形式趋向不规则活动。结果提示5-HT能递质系统对mPFC神经元活动具有重要的调节作用。

关键词： 内侧前额叶皮层; 5,7-双羟色胺; 锥体神经元; 中间神经元; 电生理学; 大鼠

分类号：Q426

Intraventricular injection of 5,7-dihydroxytryptamine alters neuronal activity of neurons in the medial prefrontal cortex of rat

FAN Ling-Ling^*, HU Zhi-Hong, ZHOU Yu-Dong, LI Qian-Qian, JIA Yan-Ru, WANG Jin-Feng, ZHAO Chun-Wei

Medical College, Henan University of Science and Technology, Luoyang 471003, China

Abstract

The present study is aimed to investigated the firing activity of pyramidal neurons and interneurons in the medial prefrontal cortex (mPFC) in rats with bilateral intraventricular injection of 5,7-dihydroxytryptamine (5,7-DHT) by using in vivo extracellular recording. The results showed that the injection of 5,7-DHT reduced the 5-hydroxytryptamine (5-HT) levels in the mPFC and dorsal raphe nucleus in the rats. The firing rate of mPFC pyramidal neurons in rats with 5,7-DHT injection was significantly higher than that of normal rats, and the firing pattern of these neurons also changed significantly towards a more burst-firing, while the injection decreased the firing rate of mPFC interneurons and changed the firing pattern of the interneurons towards a more irregular. These results indicate that the lesions of the serotonergic neurons lead to the changes in the firing activity of mPFC pyramidal neurons and interneurons, suggesting that serotonergic system plays an important role in the regulation of the neuronal activity in the mPFC.

Key words: medial prefrontal cortex; 5,7-hihydroxytryptamine; pyramidal neuron; interneuron; electrophysiology; rat

收稿日期：2014-05-09　　录用日期：2014-07-15

通讯作者：范玲玲　　E-mail: emmafll@163.com

引用本文：

范玲玲, 胡志红, 周玉东, 李倩倩, 贾焱如, 王金峰, 赵春伟. 5,7-双羟色胺侧脑室注射对大鼠内侧前额叶皮层神经元兴奋性的影响[J]. 生理学报 2014; 66 (6): 667-674.

FAN Ling-Ling, HU Zhi-Hong, ZHOU Yu-Dong, LI Qian-Qian, JIA Yan-Ru, WANG Jin-Feng, ZHAO Chun-Wei. Intraventricular injection of 5,7-dihydroxytryptamine alters neuronal activity of neurons in the medial prefrontal cortex of rat. Acta Physiol Sin 2014; 66 (6): 667-674 (in Chinese with English abstract).