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吗啡诱导大鼠穿梭觅药状态下内侧前额皮层边缘前区遥测脑电小波包提取及熵分析

白羽, 白家明, 李晶, 李敏, 虞冉, 潘群皖

皖南医学院计算机教研室；生理学教研室，芜湖 241000

摘要

本研究旨在研究内侧前额皮质(medial prefrontal cortex, mPFC)边缘前区(prelimbic cortical area, PL)遥测脑电的特征性改变与觅药行为之间的关系。将PL区埋藏脑立体定位电极的大鼠分成手术对照组和吗啡诱导组，后者制作吗啡诱导条件性位置偏爱(conditioned place preference, CPP)模型。利用CPP视频系统结合脑电无线遥测技术，记录两组大鼠在黑-白箱穿梭和白-黑箱穿梭时大鼠PL区实时脑电，采用小波包提取、Welch谱功率算法、归一化幅值和Shannon熵分析技术，对所测原始脑电波进行综合分析。结果显示，与手术对照组比较，吗啡诱导CPP组大鼠黑-白箱穿梭潜伏期，左侧PL区小波提取脑电各频段平均波幅减小，脑电成分在10~50 Hz频段Welch谱功率强度显著增加(P < 0.01或P < 0.05)，脑电β、γ1、γ2波Shannon熵值升高(P < 0.01或P < 0.05)，平均信息熵降低(P < 0.01)。上述结果提示，吗啡诱导CPP组大鼠黑-白箱穿梭时，左侧PL区脑电各频段大幅波减少，脑电10~50 Hz频率Welch谱功率强度增加，β、γ1和γ2波Shannon熵值升高；这些变化可能与大鼠觅药意识的形成和即将发动的觅药行为产生有关。

关键词： 吗啡; 条件性位置偏爱; 边缘前区; 小波包提取; Shannon熵

分类号：R3

[Wavelet packet extraction and entropy analysis of telemetry EEG from the prelimbic cortex of medial prefrontal cortex in morphine-induced CPP rats.] [Article in Chinese]

BAI Yu, BAI Jia-Ming, LI Jing, LI Min, YU Ran, PAN Qun-Wan

Department of Computer； Department of Physiology, Wannan Medical College, Wuhu 241000, China

Abstract

The purpose of the present study is to analyze the relationship between the telemetry electroencephalogram (EEG) changes of the prelimbic (PL) cortex and the drug-seeking behavior of morphine-induced conditioned place preference (CPP) rats by using the wavelet packet extraction and entropy measurement. The recording electrode was stereotactically implanted into the PL cortex of rats. The animals were then divided randomly into operation-only control and morphine-induced CPP groups, respectively. A CPP video system in combination with an EEG wireless telemetry device was used for recording EEG of PL cortex when the rats shuttled between black-white or white-black chambers. The telemetry recorded EEGs were analyzed by wavelet packet extraction, Welch power spectrum estimate, normalized amplitude and Shannon entropy algorithm. The results showed that, compared with operation-only control group, the left PL cortex's EEG of morphine-induced CPP group during black-white chamber shuttling exhibited the following changes: (1) the amplitude of average EEG for each frequency bands extracted by wavelet packet was reduced; (2) the Welch power intensity was increased significantly in 10-50 Hz EEG band (P < 0.01 or P < 0.05); (3) Shannon entropy was increased in β, γ₁, and γ₂ waves of the EEG (P < 0.01 or P < 0.05); and (4) the average information entropy was reduced (P < 0.01). The results suggest that above mentioned EEG changes in morphine-induced CPP group rat may be related to animals' drug-seeking motivation and behavior launching.

Key words: Morphine; conditioned place preference; prelimbic cortex; wavelet packet extraction; Shannon entropy

收稿日期：2014-05-14　　录用日期：2014-07-24

通讯作者：潘群皖　　E-mail: panqunw@163.com

引用本文：

白羽, 白家明, 李晶, 李敏, 虞冉, 潘群皖. 吗啡诱导大鼠穿梭觅药状态下内侧前额皮层边缘前区遥测脑电小波包提取及熵分析[J]. 生理学报 2014; 66 (6): 675-682.

BAI Yu, BAI Jia-Ming, LI Jing, LI Min, YU Ran, PAN Qun-Wan. [Wavelet packet extraction and entropy analysis of telemetry EEG from the prelimbic cortex of medial prefrontal cortex in morphine-induced CPP rats.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (6): 675-682 (in Chinese with English abstract).