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激动素对衰老大鼠免疫功能及其脾淋巴细胞体外增殖的影响

李梦云, 欧阳五庆, 吴小利, 郑寅, 高瑞, 汤佳莘

西北农林科技大学动物医学院，杨陵 712100；河南科技大学动物科技学院，洛阳 471003

摘要

本研究旨在探讨激动素对D-半乳糖(D-gal)致衰老大鼠免疫功能的保护作用及其对脾淋巴细胞体外增殖的影响。50只SD大鼠随机分为5组，分别为青年对照组、衰老模型组、激动素低剂量组、激动素中剂量组和激动素高剂量组。除青年对照组外，其它组大鼠颈背部每天皮下注射125 mg/kg D-gal，持续45 d，制造衰老模型。从第11天开始，激动素低、中、高剂量组分别每天用5、10、20 mg/kg激动素溶液灌胃给药。检测血清IgG、IgA、IgM含量，取脾组织制备脾淋巴细胞悬液，检测体外脾淋巴细胞刺激指数(stimulation index, SI)、细胞凋亡率和细胞增殖指数(proliferation index, PI)等。结果显示，衰老模型组三种免疫球蛋白含量、SI和PI都较青年对照组降低，细胞凋亡率显著升高。激动素处理组血清免疫球蛋白含量、PI、SI较模型组升高，细胞凋亡较模型组降低，其中高剂量组这几项指标与青年对照组比较差异不显著。以上结果提示，激动素能有效抑制脾淋巴细胞的凋亡，刺激细胞增殖，从而提高免疫力，延缓机体的衰老。

关键词： 激动素; 脾; 抗衰老; 免疫力; 增殖

分类号：Q255

[Effect of kinetin on immunity and splenic lymphocyte proliferation in vitro in D-galactose-induced aging rats.] [Article in Chinese]

LI Meng-Yun, OUYANG Wu-Qin, WU Xiao-Li, ZHENG Yi, GAO Rui, TANG Jia-Xin

College of Veterinary Medicine, Northwest A&；F University, Yangling 712100, China； College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China

Abstract

The purpose of this paper is to study the effect of kinetin (Kn) on immunity and splenic lymphocyte proliferation in vitro of aging rats induced by D-galactose (D-gal). Fifty SD rats were randomly divided into five groups: control group, aging model group, Kn low dose group, Kn middle dose group and Kn high dose group. The aging model group was proposed by napes subcutaneous injection of D-gal (125 mg/kg) for 45 d, and anti-aging groups were intragastrically administered with 5, 10, 20 mg/kg of Kn respectively from day 11. IgG, IgA, IgM contents of serum, the apoptosis percentage, stimulation index (SI) and proliferation index (PI) of splenic lymphocyte in vitro were evaluated. The results showed that the apoptosis percentage of splenic lymphocyte in aging model rats was higher, the serum IgG, IgA and IgM contents, SI and PI were lower than control group. Kn significantly decreased the apoptosis percentage of splenic lymphocyte, while increased the serum IgG, IgA and IgM contents, SI and PI in aging model group. These results suggest that Kn could inhibit the apoptosis, while promote the proliferation of splenic lymphocyte, and then effectively enhance the immune power of the aging rats and slow down the aging process.

Key words: kinetin; spleen; anti-aging; immunity; proliferation

收稿日期：2014-01-18　　录用日期：2014-04-17

通讯作者：欧阳五庆　　E-mail: oywq506@sina.com

引用本文：

李梦云, 欧阳五庆, 吴小利, 郑寅, 高瑞, 汤佳莘. 激动素对衰老大鼠免疫功能及其脾淋巴细胞体外增殖的影响[J]. 生理学报 2014; 66 (5): 605-611.

LI Meng-Yun, OUYANG Wu-Qin, WU Xiao-Li, ZHENG Yi, GAO Rui, TANG Jia-Xin. [Effect of kinetin on immunity and splenic lymphocyte proliferation in vitro in D-galactose-induced aging rats.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (5): 605-611 (in Chinese with English abstract).