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TRPV1受体基因敲除雌性小鼠背根神经节P2X3受体表达升高

方潇, 时霄寒, 黄丽斌, 戎伟芳, 马蓓

中国人民解放军第二军医大学生理学教研室，上海 200433；上海交通大学医学院生理学教研室，上海 200003

摘要

本文旨在考察瞬时受体电位香草酸亚型1 (transient receptor potential vanilloid 1, TRPV1受体)基因敲除后小鼠慢性炎症条件下机械痛阈的改变。通过足底注射给予完全弗氏佐剂(20 μL)介导雌性小鼠慢性炎症痛的形成，利用弗莱毛测痛法测量TRPV1受体基因敲除型及野生型雌性小鼠在给药前1天和给药后8天内的机械痛阈。给药后第9天处死小鼠，利用蛋白质免疫印迹技术研究两组小鼠脊髓背根神经节(dorsal root ganglion, DRG)和脊髓背角中c-Fos蛋白和P2X3受体表达的差别。结果显示，与野生型小鼠相比，TRPV1受体基因敲除型小鼠基础机械痛阈明显增高(P < 0.05)；足底注射CFA后第3天起，TRPV1受体基因敲除型小鼠机械痛阈高于野生型小鼠(P < 0.05)；蛋白质免疫印迹结果表明TRPV1受体基因敲除型小鼠DRG和脊髓背角中c-Fos蛋白的表达明显低于野生型小鼠(P < 0.01, P < 0.05)，TRPV1受体基因敲除型小鼠DRG中P2X3受体的表达明显高于野生型小鼠(P < 0.05)。以上结果证明TRPV1受体可能通过调节DRG和背角中的c-Fos蛋白的表达以及影响P2X3受体在DRG中的表达从而影响外周机械痛阈。

关键词： TRPV1阳离子通道; P2X3受体; 基因敲除小鼠; 背根神经节; c-Fos蛋白; 痛阈

分类号：R338.3

[Upregulation of P2X3 receptor in dorsal root ganglion of TRPV1 knockout female mice.] [Article in Chinese]

FANG Xiao, SHI Xiao-Han, HUNG Li-Bin, RONG Wei-Fang, MA Bei

Department of Physiology, Secondary Military Medical University, Shanghai 200433, China； Department of Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200003, China

Abstract

The study was aimed to investigate the changes in mechanical pain threshold in the condition of chronic inflammatory pain after transient receptor potential vanilloid 1 (TRPV1) gene was knockout. Hind-paw intraplantar injection of complete freund's adjuvant (CFA, 20 μL) produced peripheral inflammation in wild-type and TRPV1 knockout female mice. The mechanical pain thresholds were measured during the 8 days after injection and pre-injection by using Von-Frey hair. Nine days after injection, mice were killed and the differences of expression of c-Fos and P2X3 receptor in the dorsal root ganglia (DRG) and spinal cord dorsal horn were examined by Western blotting between the two groups. Compared with that in wild-type mice, the mechanical pain threshold was increased significantly in TRPV1 knockout mice (P < 0.05); 3 days after CFA injection, the baseline mechanical pain threshold in the TRPV1 knockout mice group was significantly higher than that in the wild-type mice group (P < 0.05); The result of Western blotting showed that the expression of c-Fos protein both in DRG and spinal cord dorsal horn of TRPV1 knockout mice group was decreased significantly compared with that in wild-type mice group (P < 0.01, P < 0.05), while the expression of P2X3 receptor in DRG of TRPV1 knockout mice group was increased significantly compared with that in wild-type mice group (P < 0.05). Our findings indicate that TRPV1 may influence the peripheral mechanical pain threshold by mediating the expression of c-Fos protein both in DRG and spinal cord dorsal horn and changing the expression of P2X3 receptor in DRG.

Key words: TRPV1 cation channels; P2X3 receptor; knockout mice; spinal ganglia; c-Fos protein; pain threshold

收稿日期：2013-11-30　　录用日期：2014-05-04

通讯作者：马蓓　　E-mail: mabei08@aliyun.com

引用本文：

方潇, 时霄寒, 黄丽斌, 戎伟芳, 马蓓. TRPV1受体基因敲除雌性小鼠背根神经节P2X3受体表达升高[J]. 生理学报 2014; 66 (4): 431-437.

FANG Xiao, SHI Xiao-Han, HUNG Li-Bin, RONG Wei-Fang, MA Bei. [Upregulation of P2X3 receptor in dorsal root ganglion of TRPV1 knockout female mice.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (4): 431-437 (in Chinese with English abstract).