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18β-甘草次酸对KCl和苯肾上腺素诱导的大鼠离体肾脏叶间动脉收缩的抑制作用

张雯, 马克涛, 王洋, 司军强, 李丽^*

新疆石河子大学医学院生理教研室/新疆地方与民族高发病教育部重点实验室，石河子，832002

摘要

本文旨在观察18β-甘草次酸(18β-glycyrrhetintic acid, 18β-GA)对KCl和苯肾上腺素(phenylephrine, PE)诱导的离体大鼠肾脏叶间动脉(renal interlobar artery, RIA)收缩的影响。急性分离大鼠RIA，应用压力肌动图技术，观察18β-GA预处理后大鼠RIA对内皮非依赖血管收缩剂KCl和PE的反应情况。用微动脉段标本的全细胞膜片钳技术，观察100 μmol/L 18β-GA对血管段上平滑肌细胞的膜电容(Cinput)、膜电导(Ginput)和膜电阻(Rinput)的影响。结果显示，KCl (30~100 mmol/L)和PE (0.1~30 μmol/L)均可以引起大鼠RIA浓度依赖的收缩；100 μmol/L的18β-GA预处理后，KCl和PE对大鼠RIA的缩血管作用明显降低(P < 0.01)。给予100 μmol/L 18β-GA后，RIA血管段上平滑肌细胞的Rinput、Ginput和Cinput与单个平滑肌细胞数值十分接近。以上结果提示，18β-GA可抑制KCl和PE对大鼠RIA的收缩作用，其机制可能涉及18β-GA对缝隙连接的抑制。

关键词： 缝隙连接; 肾脏叶间动脉; 18β-甘草次酸; 压力肌动图技术

分类号：R331.3

[Inhibitory effect of 18β-glycyrrhetinic acid on KCl- and PE-inducedconstriction of rat renal interlobar artery in vitro.] [Article in Chinese]

ZHANG Wen, MA Ke-Tao, WANG Yang, SI Jun-Qiang, LI Li^*

Department of Physiology/Key Laboratory of Education Ministry of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi 832002,China

Abstract

The aim of the present study is to investigate the effect of 18β-glycyrrhetinic acid (18β-GA) on KCl- and PE-induced constriction of rat renal interlobar artery (RIA). Pressure myograph system was used to observe the constriction induced by KCl and PE (endothelial independent vasoconstrictor) in acutely separated RIA of Wistar rats with or without 18β-GA pretreatment. Whole-cell patch clamp recordings were used to observe the effect of 18β-GA on membrane input capacitance (C_input), membrane input conductance (G_input) or membrane input resistance (R_input) of smooth muscle cells embedded in arteriole segment. The results showed that both KCl (30-100 mmol/L) and PE (0.1-30 μmol/L) induced contraction of RIA in a concentration-dependent way. After pretreatment with 18β-GA (100 μmol/L), KCl- or PE-induced constriction of RIA was significantly decreased. After application of 18β-GA (100 μmol/L), the C_input, G_input and R_input of the in situ smooth muscle cells were very close to those of dispersed single smooth muscle cells. These results suggest 18β-GA inhibits the contraction induced by KCl and PE, and the underlying mechanism may involve the inhibitory effect of 18β-GA on gap junction.

Key words: Gap junction; renal interlobar artery; 18β-glycyrrhetinic acid; pressure myograph system

收稿日期：2013-08-19　　录用日期：2013-12-16

通讯作者：李丽　　E-mail: Lily7588@163.com

引用本文：

张雯, 马克涛, 王洋, 司军强, 李丽. 18β-甘草次酸对KCl和苯肾上腺素诱导的大鼠离体肾脏叶间动脉收缩的抑制作用[J]. 生理学报 2014; 66 (2): 195-202.

ZHANG Wen, MA Ke-Tao, WANG Yang, SI Jun-Qiang, LI Li. [Inhibitory effect of 18β-glycyrrhetinic acid on KCl- and PE-inducedconstriction of rat renal interlobar artery in vitro.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (2): 195-202 (in Chinese with English abstract).