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鞘氨醇-1-磷酸受体在心肌缺血和缺血再灌注后的差异表达

张更谦^*, 梁正, 张晓嘉

山西医科大学法医学院，太原 030001

摘要

鞘氨醇-1-磷酸(sphingosine-1-phosphate, S1P)是心肌病的重要调节分子和生物标记。我们假设S1P受体表达参与了S1P对心肌的保护作用并可作为缺血性心肌病的标记。通过在体结扎大鼠左冠状动脉前降支，建立大鼠手术心肌缺血和心肌缺血再灌注模型，在心肌缺血早期不同时间段(15 min，30 min，1 h)和缺血40 min再灌注2 h后用实时定量PCR方法检测S1PR1、S1PR2、S1PR3在心肌中的表达。结果显示，缺血再灌注组心肌中S1PR3的mRNA表达较假手术组明显升高，而S1PR1和S1PR2 表达无变化；各缺血组心肌中S1P的三种受体mRNA表达与假手术组相比无统计学差异。结果提示：S1P受体不适合作为早期心肌缺血的标记物；S1P受体在心肌缺血和缺血再灌注时有不同的表达模式，S1P浓度变化和S1P受体表达改变的联合作用共同构成了在心肌缺血和缺血再灌注后的调控网络。

关键词： 鞘氨醇-1-磷酸受体; 心肌缺血; 心肌缺血再灌注; 实时定量PCR

分类号：R542.2

Sphingosine-1-phosphate receptors respond differently to early myocardial ischemia and ischemia-reperfusion in vivo

ZHANG Geng-Qian^*, LIANG Zheng, ZHANG Xiao-Jia

School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China

Abstract

Sphingosine-1-phosphate (S1P) has been demonstrated to be a mediator and marker of heart diseases. We hypothesized that the expression of S1P receptors is involved in the S1P-mediated cardioprotection in vivo and may serve as a biomarker of ischemic heart disease. In vivo models of myocardial ischemia (MI) and ischemia-reperfusion (IR) were established by ligation of the left anterior descending artery (LAD) of rat heart, the mRNA expressions of S1PR1-3 were detected using real time PCR at different time intervals after ischemia (LAD for 15 min, 30 min, and 1 h) and IR. The results showed that mRNA expression of S1PR3, but not S1PR1 and S1PR2, increased greatly after IR. No statistical difference was found in any of the three S1P receptors after MI within 1 h. Regarding the studies of lipid concentration changes in myocardiopathy, we conclude that S1P receptors are not early response biomarkers for MI. There are different mechanisms when S1P plays a protection role in heart during MI and IR. The cooperation of lipid content and S1P receptor expression appears to form a regulation network during MI and IR.

Key words: sphingosine-1-phosphate receptor; myocardial ischemia; ischemia-reperfusion; real time PCR

收稿日期：2013-06-06　　录用日期：2013-11-19

通讯作者：张更谦　　E-mail: zgengqian@163.com

引用本文：

张更谦, 梁正, 张晓嘉. 鞘氨醇-1-磷酸受体在心肌缺血和缺血再灌注后的差异表达[J]. 生理学报 2014; 66 (2): 169-174.

ZHANG Geng-Qian, LIANG Zheng, ZHANG Xiao-Jia. Sphingosine-1-phosphate receptors respond differently to early myocardial ischemia and ischemia-reperfusion in vivo. Acta Physiol Sin 2014; 66 (2): 169-174 (in Chinese with English abstract).