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成年雄性斑胸草雀高级发声中枢-弓状皮质栎核突触的长时程可塑性

李凤灵, 李东风^*

华南师范大学生命科学学院，广东省高等学校生态与环境科学重点实验室，广州 510631

摘要

长时程突触可塑性是学习与记忆神经机制的重要组成部分。雄性斑胸草雀的习得性发声与高级发声中枢(high vocal center, HVC)-弓状皮质栎核(robust nucleus of the arcopallium, RA)通路密切相关，而HVC-RA突触的长时程可塑性表现尚不清楚。本文应用在体电生理方法研究了成年雄性斑胸草雀HVC-RA突触的长时程可塑性。结果显示，(1)生理性刺激(δ节律刺激)和低频刺激(3 Hz, 3 min)并不能诱导出长时程可塑性，前者不引起任何诱发群体峰电位幅度的变化，而后者可引起配对脉冲诱发的第二个群体峰电位幅度的短时程抑制(short-term depression, STD)；(2)高频刺激(400 Hz, 2 s)可引起诱发群体峰电位幅度的长时程抑制(long-term depression, LTD)。上述结果提示，LTD是成年雄性斑胸草雀HVC-RA突触长时程可塑性的表现形式，是发声这一感知运动的记忆机制之一，在一定程度上可以解释成年鸟鸣唱的可塑性。

关键词： 雄性斑胸草雀; HVC-RA突触; 长时程突触可塑性

分类号：R338

[Long-term plasticity of HVC-RA synapses in adult male zebra finches.] [Article in Chinese]

LI Feng-Ling, LI Dong-Feng^*

School of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Higher Education of Guangdong Province, Guangzhou 510631, China

Abstract

Long-term synaptic plasticity is considered as a key part of the neural mechanism of learning and memory. The production of learned vocalization of male zebra finches is closely related to high vocal center (HVC)-robust nucleus of the arcopallium (RA) pathway. However, the long-term plasticity of HVC-RA synapses is unclear. This study investigated the long-term plasticity of HVC-RA synapses in adult male zebra finches through in vivo field potential recording. The results showed that physiologic stimulation, i.e., δ rhythmic stimulation and low frequency stimulation could not effectively induce long-term synaptic plasticity. The former leaded to no change of the amplitudes of evoked population spikes, and the latter induced short-term depression (STD) of the amplitudes of the second evoked population spikes caused by paired pulses. But high frequency stimulation induced long-term depression (LTD) of the amplitudes of evoked population spikes to show out long-term synaptic plasticity. These results suggest that LTD represents the long-term plasticity of HVC-RA synapses in adult male zebra finches, which may be a key part of the neural mechanism of vocal learning and memory and can explain the plasticity of adult song to some degree.

Key words: male zebra finches; HVC-RA synapses; long-term synaptic plasticity

收稿日期：2013-07-21　　录用日期：2013-10-10

通讯作者：李东风　　E-mail: dfliswx@126.com

引用本文：

李凤灵, 李东风. 成年雄性斑胸草雀高级发声中枢-弓状皮质栎核突触的长时程可塑性[J]. 生理学报 2013; 65 (6): 586-592.

LI Feng-Ling, LI Dong-Feng. [Long-term plasticity of HVC-RA synapses in adult male zebra finches.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (6): 586-592 (in Chinese with English abstract).