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内源性一氧化碳在大鼠败血症性休克时低血压发生机制中的作用

欧和生杨 军 董林旺 庞永政 苏静怡 唐朝枢 刘乃奎

北京医科大学心血管研究所, 北京100083

摘要

应用盲肠结扎法制备大鼠败血症休克模型, 研究内源性一氧化碳( CO) 在败血症休克时低血压发病中的作用。用血红素加氧酶(heme ox ygenase, HO)抑制剂2, 4-二甘油次卟啉锌( zinc deuteroporphyrin 2, 4-bisgly col, ZnDPBG) 处理大鼠后, 观察动物动脉血压, 同时测定主动脉平滑肌组织中HO 活性和CO生成量。结果发现: 败血症大鼠动脉收缩压、舒张压降低,同时血管平滑肌HO 活性和CO生成明显增加。败血症大鼠用ZnDPBG处理后, 动脉血压明显回升, 同时HO 活性和CO生成明显被抑制。实验表明败血症休克时低血压的发生与血管平滑肌细胞HO活性增加和内源性CO 生成增多明显相关; 应用HO抑制剂阻断HO活性能导致内源性CO生成减少, 继而使败血症休克时大鼠血压明显回升。实验提示, 内源性CO对血管张力具有重要的调节作用; HO 活性和内源性CO 生成增加是败血症休克时低血压发生的重要机制之一。

关键词： 低血压; 败血症休克; 一氧化碳; 血红素加氧酶

Role of endogenous carbon monoxide in the pathogenesis of hypotension during septic shock

OU He-Sheng, Yang Jun, DONG Lin-Wang, PANG Yong-Zheng, SU Jing-Yi, TANG Chao-Shu, LIU Nai-Kui

Institute of Cardiovascular Research, Beijing Medical University , Bejing 100083, China

Abstract

A sepsis model induced by cecal ligation and puncture was used to study the role of endogenouscarbon monoxide in hy potension patho genesis of r ats during septic shock. After administr at ion ofzinc deuteroporphyr in 2, 4- bisglycol ( ZnDPBG) , an inhibitor o f heme oxyg enase ( HO) , bloo d pressure( BP) , HO activity and carbon monoxide ( CO) r elease from v ascular muscle tissue were measured. Theresults showed that BP of sepsis rats, including systolic and diastolic arterial BP, decreased significantlywhile HO activ ity and CO content w ere significantly increased. In contrast, after administr at ion of ZnDPBG,BP of sepsis rats was significantly increased while the HO activity and CO pr oduction w ere signif-icantly decr eased. These findings suggest that HO activ ity and CO r elease within vascular musculature areincreased during septic shock; inhibition of HO may elevate BP of rats dur ing septic shock t hrough a decreaseof endogenous CO production. It is co ncluded that endo genous CO derived from v ascular musclecells plays an important role in regulating vascular tone, and the up- regulation of HO act ivity followed bysubsequent CO production contributes to hypotension pat hogenesis during septic shock.

Key words: hypotension; septic shock; carbonmonoxide; hemeoxygenase

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引用本文：

欧和生杨 军 董林旺 庞永政 苏静怡 唐朝枢 刘乃奎. 内源性一氧化碳在大鼠败血症性休克时低血压发生机制中的作用[J]. 生理学报 1999; 51 (1): 1-6.

OU He-Sheng, Yang Jun, DONG Lin-Wang, PANG Yong-Zheng, SU Jing-Yi, TANG Chao-Shu, LIU Nai-Kui. Role of endogenous carbon monoxide in the pathogenesis of hypotension during septic shock. Acta Physiol Sin 1999; 51 (1): 1-6 (in Chinese with English abstract).