过刊浏览

子宫珠蛋白结合蛋白在antiflammin-1促进LPS诱导的RAW264.7细胞IL-10表达和分泌中的作用

张天杰, 汉建忠, 刘惠君, 廖晓红, 李晨, 罗自强

中南大学湘雅基础医学院生理学系,长沙 410078；湘南学院生理学教研室.郴州 423000；长治医学院生理教研室,长治 046000

摘要

本文旨在观察antiflammin-1 (AF-1)预处理对脂多糖(lipopolysaccharide, LPS)诱导的RAW264.7细胞白细胞介素10 (interleukin-10, IL-10)分泌的影响，并探讨子宫珠蛋白结合蛋白(uteroglobin-binding protein, UGBP)在介导AF-1对LPS诱导的巨噬细胞IL-10分泌及表达影响中的作用。将RAW264.7细胞分为空白对照组、LPS组(1 µg/mL LPS)、AF-1组(100 μmol/L AF-1)、AF-1+LPS组(100 μmol/L AF-1预处理2 h后，加入LPS)及抗UGBP抗体+AF-1+LPS组(抗-UGBP抗体预处理30 min后，再依次用AF-1和LPS处理)，用ELISA法、RT-PCR法分别检测各组IL-10蛋白分泌和mRNA表达。结果显示，AF-1预处理呈剂量依赖性显著增加LPS诱导的RAW264.7细胞IL-10分泌，并上调IL-10 mRNA水平；而抗UGBP抗体预处理可显著抑制AF-1的促IL-10分泌和上调mRNA表达作用。以上结果提示，AF-1可促进LPS诱导的巨噬细胞IL-10的分泌和mRNA表达，且该作用依赖于UGBP的介导。

关键词： 抗炎素-1; 子宫珠蛋白; 脂多糖; 白细胞介素10

分类号：R392.11

[Role of UGBP in Antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin.] [Article in Chinese]

ZHANG Tian-Jie, HAN Jian-Zhong, LIU Hiu-Jun, LIAO xiao-Hong, LI Chen, LUO Zi-Qiang

1Department of Physiology, Central South University, Changsha 410078, China； 2Department of Physiology, Xiangnan College, Chenzhou 423000, China； 3Department of Physiology, Changzhi Medical College, Changzhi 046000, China

Abstract

The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secretion from macrophages, and this effect is mediated by UGBP.

Key words: antiflammin-1; uteroglobin; lipopolysaccharide; interleukin-10

收稿日期：2013-01-17　　录用日期：2013-07-02

通讯作者：李晨,罗自强　　E-mail: luozq1962@163.com, ivyandl.li@hotmail.com

引用本文：

张天杰, 汉建忠, 刘惠君, 廖晓红, 李晨, 罗自强. 子宫珠蛋白结合蛋白在antiflammin-1促进LPS诱导的RAW264.7细胞IL-10表达和分泌中的作用[J]. 生理学报 2013; 65 (4): 363-369.

ZHANG Tian-Jie, HAN Jian-Zhong, LIU Hiu-Jun, LIAO xiao-Hong, LI Chen, LUO Zi-Qiang. [Role of UGBP in Antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (4): 363-369 (in Chinese with English abstract).