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收缩活动促进新生大鼠培养心室肌细胞的~3H-亮氨酸掺入和细胞生长

丁小凌, 周承愉, 李云霞

湖南医科大学心血管生理研究室； 湖南医科大学心血管生理研究室，长沙 410078

摘要

本工作应用培养的新生大鼠心室肌细胞,以~3H-亮氨酸掺入、细胞直径和细胞体积为指标,观察了收缩活动对心肌蛋白质合成速率及细胞生长的影响。在含10％血清的培养介质中,常钾搏动组的心肌细胞(CMC)的~3H-亮氨酸掺入显著高于高钾停搏组的心肌细胞(QMC),24h的掺入量分别为1229±29与1076±60cpm/10~5细胞(每组n=5,P<0.01),CMC掺入为QMC的114.2％。QMC组细胞直径和体积分别为15.14±0.42μm和1842±123μm~3,而CMC组为16.82±0.64μm和2495±210μm~3,分别比前者高11.1％和35.5％(P<0.01,每组n=6)。这些变化随培养时间的延长更趋明显,但两组间的细胞数目并无显著差异(P>0.05)。上述结果表明:收缩活动本身能促进心肌细胞蛋白质合成和细胞生长。

关键词： 心肌细胞培养; 收缩活动; 蛋白质合成速率; 细胞生长; 大鼠

Contraction stimulates incorporation of 3H-leucine and cell growth in cultured neonatal rat ventricular myocytes

DING XIAO-LING, ZHOU CHENG-YU , LI YUN-XIA

Laboratory of Cardiovascular Physiology, Hunan Medical University, Changsha 410078

Abstract

To determine whether contraction could influence cell growth, the rate of protein synthesis (3H-leucine incorporation) and cell diameter and volume were measured in cultured neonatal rat cardiac myocytes beating spontaneously or arrested by high potassium. In medium supplemented with 10% calf serum, the 3H-leucine incorporation for 24 h in contracting myocytes (CMC) was significantly higher by 14.2% than that in quiescent myocytes (QMC), i.e. 1,229 +/- 29 cpm/10(5) cells vs. 1,076 +/- 60 cpm/10(5) cells (P < 0.01, n = 5 for each group). The cell diameter and cell volume in QMC group were respectively 15.14 +/- 0.42 microns and 1,842 +/- 123 microns3, while in the CMC group the corresponding figures reached to 16.82 +/- 0.64 microns3 and 2,495 +/- 210 microns3, increased by 11.1% and 35.5% respectively (P < 0.01, n = 6 for each group). With prolongation of culture time, the differences in these parameters between CMC and QMC became even more significant. In all these experiments, there was no significant difference in cell number between the two groups (P > 0.05). It is concluded that contraction per se can accelerate protein synthesis and cell growth in neonatal rat ventricular myocardium.

Key words: contraction; cardiac myocyte culture; rate of protein synthesis; cell growth; rat

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引用本文：

丁小凌, 周承愉, 李云霞. 收缩活动促进新生大鼠培养心室肌细胞的~3H-亮氨酸掺入和细胞生长[J]. 生理学报 1992; 44 (6): 591-596.

DING XIAO-LING, ZHOU CHENG-YU , LI YUN-XIA. Contraction stimulates incorporation of 3H-leucine and cell growth in cultured neonatal rat ventricular myocytes. Acta Physiol Sin 1992; 44 (6): 591-596 (in Chinese with English abstract).