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电刺激延髓最后区对血浆肾素活性及肾交感神经活动的影响

邱慧, 柴象枢

山东省医学科学院生理室，济南 250001

摘要

实验在68只家兔上进行。乌拉坦麻醉。用RM-6000型多道仪同步记录呼吸(Respir)、血压(BP)、心率(HR)及肾神经放电(RSNA)。用放免法测定血浆肾素活性(PRA)。应用外径0.3mm的双芯同心电极置于延髓最后区(AP)处进行电刺激,每30s刺激4s。实验分为三组。第一组仅刺激AP观察到PRA增加了91％,RSNA出现以兴奋为主的三种放电形式,BP升高,HR减慢,呼吸无显著变化。第二组去除双侧肾神经后再刺激AP,PRA增加甚微,RSNA及血流动力学反应与第一组刺激前后的变化相似。第三组注射心得安后,刺激AP,这时除PRA明显抑制外,RSNA、BP、HR及呼吸亦与第一、第二组刺激前后的变化相似。上述结果表明电刺激兔AP能引起肾素释放增加,肾交感神经放电及血流动力学活动增强。

关键词： 最后区; 血浆肾素活性; 肾交感神经活动; 血流动力学

Effect of electrical stimulation of the area postrema on plasma renin activity and renal sympathetic nerve activity in rabbits

QIU Hui, CHAI Xiang-Shu

Department of Physiology,Institute of Basic Medicine, Shandong Medical Acadzmy, Jinan 250001, China

Abstract

68 urethan-anesthetized rabbits were prepared for registration of changes of respiration, arterial blood pressure (BP), heart rate (HR) and renal sympathetic nerve activity (RSNA) due to stimulation of area postrema (AP) by rectangular pulse trains each lasting for 4 s for every 30 s. During 40 min of such a stimulation paradigm the venous blood samples were collected for radioimmunoassay of plasma renin activity (PRA) (both pre- and post-stimulation), RSNA registered and processed by a computer. Animals were divided into three groups: (1) with AP stimulation only (n = 47); (2) AP stimulation after bilateral renal denervation (n = 13); (3) AP stimulation after propranolol injection (n = 8). In Group I, a 91% increase in PRA, an augmentation of RSNA, a rise of BP and a decrease of HR were observed, while respiration did not show obvious change. In Group II, hemodynamic and RSNA response was similar to that in Group I, but PRA was not changed significantly. In Group III, the effects on BP, HR, respiration and RSNA showed no remarkable changes compared with Group I, but significant inhibition of the response of PRA [from 0.65 +/- 0.07 ng/(ml.h-1) to 0.72 +/- 0.10 ng/(ml.h-1), P > 0.05] was observed. The results mentioned above suggested that electrical stimulation of AP may induce an increase in renin release and renal sympathetic nerve activity and hemodynamic changes in rabbits.

Key words: Area postrema; electrical stimulation; plasma renin activity; renal sympathetic nerve activity

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引用本文：

邱慧, 柴象枢. 电刺激延髓最后区对血浆肾素活性及肾交感神经活动的影响[J]. 生理学报 1992; 44 (6): 569-575.

QIU Hui, CHAI Xiang-Shu. Effect of electrical stimulation of the area postrema on plasma renin activity and renal sympathetic nerve activity in rabbits. Acta Physiol Sin 1992; 44 (6): 569-575 (in Chinese with English abstract).