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高原鼠兔和Sprague-Dawley大鼠离体主动脉环对低氧的收缩反应

马爽, 马燕, 格日力^*

青海大学医学院高原医学研究中心，西宁 810001

摘要

本文旨在观察低氧对Sprague-Dawley (SD)大鼠和高原鼠兔(Ochotona curzoniae)离体主动脉环张力的影响。分别制备SD大鼠和高原鼠兔主动脉环，采用离体血管环灌流方法，经生物信号采集与分析系统测定不同药物或1 h低氧处理对内皮完整和去内皮血管环张力的影响。结果显示，SD大鼠和高原鼠兔主动脉环对高钾(80 mmol/L KCl)的收缩反应之间无明显差异。SD大鼠内皮完整的主动脉环对ACh有明显的舒张反应，去内皮的主动脉环则对ACh没有舒张反应；与SD大鼠相反，无论是否去内皮，预收缩的高原鼠兔主动脉环经ACh (1 μmol/L)处理后出现收缩反应。无论是否去内皮，有钙或无钙的Kreb’s液孵育，高原鼠兔主动脉环低氧处理后的收缩率均明显高于SD大鼠。以上结果提示，高原鼠兔和SD大鼠相比，主动脉对低氧的收缩反应更敏感，而这个增强的反应主要依赖于主动脉平滑肌细胞钙库的内钙释放。

关键词： 高原鼠兔; Sprague-Dawley 大鼠; 胸主动脉环; 血管收缩; 低氧

分类号：Q494

[Contraction responses of isolated aortic rings of pika (Ochotona curzoniae) and Sprague-Dawley rat to hypoxia.] [Article in Chinese]

MA Shuang, MA Yan, GE Ri-Li^*

Research Center for High Altitude Medicine，Medical College of Qinghai University, Xining 810001, China

Abstract

The aim of the present study was to observe the effects of hypoxia on tensions of aortic rings of pika (Ochotona curzoniae) and Sprague-Dawley (SD) rat. The aortic rings were prepared, and in vitro vascular ring perfusion was used to assay the effects of hypoxia or different drugs on contraction responses of the rings with or without endothelium. The results showed that, there was no difference of the contractions to KCl (80 mmol/L) between the aortic rings of the pikas and SD rats. After pre-contraction with NE (1 μmol/L), the aortic rings with endothelium of the SD rats showed obvious relaxation to ACh (1 μmol/L), whereas the aortic rings of the pikas, no matter with or without endothelium, showed significant and unusual contraction to ACh. The aortic rings of pikas, no matter with or without endothelium, exhibited greater contraction when treated by 1 h of hypoxia, compared with those in SD rats; The similar result was showed under hypoxia in combination with Ca(2+) removal. These results suggest that the contraction response to hypoxia in pika is more sensitive compared to that in SD rat, which is dependent on the release of calcium from intracellular calcium store.

Key words: pika; Sprague-Dawley rat; aortic ring; vascular contraction; hypoxia

收稿日期：2012-08-03　　录用日期：2012-11-19

通讯作者：格日力　　E-mail: geriligao@hotmail.com

引用本文：

马爽, 马燕, 格日力. 高原鼠兔和Sprague-Dawley大鼠离体主动脉环对低氧的收缩反应[J]. 生理学报 2013; 65 (2): 122-128.

MA Shuang, MA Yan, GE Ri-Li. [Contraction responses of isolated aortic rings of pika (Ochotona curzoniae) and Sprague-Dawley rat to hypoxia.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (2): 122-128 (in Chinese with English abstract).