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孕期酒精暴露对视网膜发育及细胞分化的影响

席艳, 周洁, 孔维芳, 王强, 刘彬, 郑红, 邓锦波^*

河南大学神经生物研究所，开封 475004

摘要

本文旨在研究酒精暴露对视网膜的发育和细胞分化过程所造成的影响。建立C57BL/6J小鼠孕期酒精暴露模型，用HE染色和免疫荧光技术，选取P0到P30仔鼠观察视网膜的正常发育和细胞分化过程，及酒精对其造成的影响。结果显示，孕期酒精暴露可以诱导仔鼠视网膜片层化形成延迟、水平细胞和双极细胞的数量减少(P < 0.05)和极性紊乱、视网膜增厚(P < 0.01)，具有酒精剂量依赖性(P < 0.05)和酒精暴露停止后的长时程效应。以上结果提示，孕期酒精暴露可引起仔鼠视觉相关神经元的数量减少及形态异常，导致视觉系统的发育障碍。

关键词： 片层化; 双极细胞; 视网膜发育; 水平细胞; 胎儿酒精综合症

分类号：R774.1

[Influence of prenatal alcohol exposure on retinal development and cell differentiation.] [Article in Chinese]

XI Yan, ZHOU Jie, KONG Wei-Fang, WANG Qiang, LIU Bin, ZHENG Hong, DENG Jin-Bo^*

Institute of Neurobiology, Henan University, Kaifeng 475004, China

Abstract

The aim of the present study was to investigate the effects of prenatal alcohol exposure (PAE) on the development and cell differentiation of retina in offspring. The mouse model of PAE was made. HE staining and immunofluorescent labeling were carried out to visualize the structure, development and cell differentiation of the retina from postnatal day 0 (P0)-P30 offspring. The results showed that PAE can lead to the retardation of retinal development, the reduction of number of bipolar cells and horizontal cells, the disorder of horizontal cells' polarity, as well as the retinal thickening in a dose-dependent manner. The data suggest that alcohol exposure during pregnancy can lead to the developmental retardation of retina and decreased number of bipolar cells and horizontal cells in the retina of offspring.

Key words: prenatal alcohol exposure; retinal development; horizontal cells; bipolar cells ;

收稿日期：2013-03-19　　录用日期：2013-05-17

通讯作者：邓锦波　　E-mail: jinbo_deng@henu.edu.cn

引用本文：

席艳, 周洁, 孔维芳, 王强, 刘彬, 郑红, 邓锦波. 孕期酒精暴露对视网膜发育及细胞分化的影响[J]. 生理学报 2013; 65 (5): 505-512.

XI Yan, ZHOU Jie, KONG Wei-Fang, WANG Qiang, LIU Bin, ZHENG Hong, DENG Jin-Bo. [Influence of prenatal alcohol exposure on retinal development and cell differentiation.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (5): 505-512 (in Chinese with English abstract).