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Changes in response properties of nociceptive dorsal horn neurons in a murine model of cancer pain

Donald A. Simone^*, Sergey G. Khasabov, Darryl T. Hamamoto

Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, 515 Delaware St. S.E., Minneapolis, Minnesota 55455, USA

摘要

Pain associated with cancer that metastasizes to bone is often severe and debilitating. A better understanding of the neuralmechanisms that mediate cancer pain is needed for the development of more effective treatments. In this study, we used an establishedmodel of cancer pain to characterize changes in response properties of dorsal horn neurons. Fibrosarcoma cells were implanted into andaround the calcaneus bone in mice and extracellular electrophysiological recordings were made from wide dynamic range (WDR) andhigh threshold (HT) dorsal horn neurons. Responses of WDR and HT neurons evoked by mechanical, heat, and cold stimuli applied tothe plantar surface of the hind paw were compared between tumor bearing mice and control mice. Mice exhibited hyperalgesia tomechanical and heat stimuli applied to their tumor-bearing hind paw. WDR neurons in tumor-bearing mice exhibited an increase inspontaneous activity, and enhanced responses to mechanical, heat, and cold stimuli as compared to controls. Our findings show thatsensitization of WDR neurons, but not HT neurons, contributes to tumor-evoked hyperalgesia.

关键词： cancer pain; dorsal horn; electrophysiology; hyperalgesia; sensitization

Changes in response properties of nociceptive dorsal horn neurons in a murine model of cancer pain

Donald A. Simone^*, Sergey G. Khasabov, Darryl T. Hamamoto

Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, 515 Delaware St. S.E., Minneapolis, Minnesota 55455, USA

Abstract

Pain associated with cancer that metastasizes to bone is often severe and debilitating. A better understanding of the neuralmechanisms that mediate cancer pain is needed for the development of more effective treatments. In this study, we used an establishedmodel of cancer pain to characterize changes in response properties of dorsal horn neurons. Fibrosarcoma cells were implanted into andaround the calcaneus bone in mice and extracellular electrophysiological recordings were made from wide dynamic range (WDR) andhigh threshold (HT) dorsal horn neurons. Responses of WDR and HT neurons evoked by mechanical, heat, and cold stimuli applied tothe plantar surface of the hind paw were compared between tumor bearing mice and control mice. Mice exhibited hyperalgesia tomechanical and heat stimuli applied to their tumor-bearing hind paw. WDR neurons in tumor-bearing mice exhibited an increase inspontaneous activity, and enhanced responses to mechanical, heat, and cold stimuli as compared to controls. Our findings show thatsensitization of WDR neurons, but not HT neurons, contributes to tumor-evoked hyperalgesia.

Key words: cancer pain; dorsal horn; electrophysiology; hyperalgesia; sensitization

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通讯作者：Donald A. Simone　　E-mail: simon003@umn.edu

引用本文：

Donald A. Simone, Sergey G. Khasabov, Darryl T. Hamamoto. Changes in response properties of nociceptive dorsal horn neurons in a murine model of cancer pain[J]. 生理学报 2008; 60 (5): 635-644.

Donald A. Simone, Sergey G. Khasabov, Darryl T. Hamamoto. Changes in response properties of nociceptive dorsal horn neurons in a murine model of cancer pain. Acta Physiol Sin 2008; 60 (5): 635-644 (in Chinese with English abstract).