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c-erbB2 在大鼠卵巢中的表达及其在原始卵泡生长启动中的作用

徐良全, 许爱霞, 黄健, 陈蔚云, 郑月慧^*

南昌大学医学院生殖研究所，南昌 330006

摘要

本文旨在研究原癌基因c-erbB2 在大鼠卵巢的表达，及其在原始卵泡启动过程中的作用。我们首先采用RT-PCR 方法证实大鼠卵巢中有c-erbB2 的表达，再体外合成其RNA 小干扰片段(small interference RNA, siRNA)，并用脂质体(Metafectene)介导转染体外培养的新生大鼠卵巢，在引发原癌基因c-erbB2 表达沉默或抑制的情况下，观察原始卵泡的生长启动情况，以及表皮生长因子(epidermal growth factor, EGF)在此过程中的作用是否发生变化。结果显示：将c-erbB2 siRNA转染原始卵泡后，c-erbB2 mRNA 水平明显下调(P<0.05)，三对siRNA 抑制率分别为49.6%、46.7%、82.6%，同时其蛋白表达也明显下调(P<0.01)。通过siRNA 抑制原癌基因c-erbB2 的表达，原始卵泡的启动、生长受到抑制；EGF 可以促进原始卵泡的启动生长，但此作用可以被c-erbB2 siRNA 阻断。以上结果提示，c-erbB2 参与原始卵泡启动生长过程的调控，并介导EGF 促进原始卵泡启动生长的作用。

关键词： 卵巢; 原始卵泡; c - erbB2; 表皮生长因子; RNA 干扰

分类号：Q492.5；R339.2+2

[The expression of proto-oncogene c-erbB2 and its role in the initiation of primordial follicle growth in rat ovary.] [Ariticle in Chinese]

XU Liang-Quan, XU Ai-Xia, HUANG Jian, CHEN Wei-Yun, ZHENG Yue-Hui^*

Department of Physiology Reproduction, Nanchang University Medical College, Nanchang 330006, China

Abstract

Little is known about the factors that control the initiation of growth of primordial follicles. The objective of the present study was to investigate the effect of c-erbB2 on the onset of primordial follicle development, and whether c-erbB2 mediates the effect of epidermal growth factor (EGF) in this process. We synthesized three pairs of siRNAs targeting the c-erbB2 mRNA and transferred them into the newborn rat ovary cultured in vitro with Metafectene. After siRNAs transfection, the efficiency of siRNAs was tested by examining c-erbB2 mRNA and protein levels. The level of c-erbB2 mRNA was reduced by 49.6%, 46.7% and 82.6% respectively after transfecting siRNA1, siRNA2 and siRNA3, and the level of ErbB2 protein also reduced remarkably after siRNA3 transfection. c-erbB2 siRNA3 significantly inhibited the primordial follicle initiation and development; EGF augmented primordial follicles formation, but the effect was abolished by c-erbB2 siRNA3. All of these results suggest that c-erbB2 plays an important role in primordial follicle development and folliculogenesis initiation, and mediates the effect of EGF on primordial follicle development.

Key words: ovary; primordial follicle; c-erbB2; epidermal growth factor; RNAi

收稿日期：2009-01-06　　录用日期：2009-09-07

通讯作者：郑月慧　　E-mail: yuehuizheng@163.com

引用本文：

徐良全, 许爱霞, 黄健, 陈蔚云, 郑月慧. c-erbB2 在大鼠卵巢中的表达及其在原始卵泡生长启动中的作用[J]. 生理学报 2009; 61 (5): 424-430.

XU Liang-Quan, XU Ai-Xia, HUANG Jian, CHEN Wei-Yun, ZHENG Yue-Hui. [The expression of proto-oncogene c-erbB2 and its role in the initiation of primordial follicle growth in rat ovary.] [Ariticle in Chinese] . Acta Physiol Sin 2009; 61 (5): 424-430 (in Chinese with English abstract).