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中性粒细胞在外源性硫化氢抗内毒素致急性肺损伤中的作用

黄新莉, 周晓红, 周君琳, 丁春华, 羡晓辉1

河北医科大学病理生理教研室，石家庄050017； 首都医科大学附属北京朝阳医院骨科，北京 100020

摘要

本文应用尾静脉注射脂多糖(lipopolysaccharide, LPS)致Sprague-Dawley 大鼠急性肺损伤(acute lung injury, ALI)模型和体外培养人血多形核中性粒细胞(polymorphonuclear neutrophil, PMN)，观察硫化氢(hydrogen sulfide, H2S)供体硫氢化钠(sodiumhydrosulfide, NaHS)对LPS 所致肺内PMN 聚集、微血管通透性及PMN 凋亡的影响。整体实验和体外实验分别设对照组、NaHS 组、LPS 组和LPS + NaHS 组，检测肺微血管通透性、肺内PMN 聚集以及PMN 凋亡情况。结果显示：(1)整体实验中，LPS 组大鼠的支气管肺泡灌洗液(bronchoalveloar lavage fluid, BALF)中蛋白含量、PMN 数量、肺组织中伊文思蓝(Evans blue)含量均明显高于假手术组(均P<0.05)，而LPS + NaHS 组上述指标均明显低于LPS 组(P<0.05, P<0.01)；(2)体外培养人血PMN，LPS 组和NaHS 组的PMN 凋亡百分率明显高于对照组(P<0.01)，LPS + NaHS 组明显高于LPS 组(P<0.01)。以上结果提示，NaHS 能够减少PMN 在肺内的聚集，在一定程度上起到抗LPS 所致的以肺微血管高通透性为特征的ALI 的作用，促进PMN 凋亡可能是NaHS 减轻PMN 在肺内聚集的机制之一。

关键词： 硫化氢; 内毒素; 肺损伤; 中性粒细胞

分类号：R363.2

[Role of polymorphonuclear neutrophil in exogenous hydrogen sulfide attenuating endotoxin-induced acute lung injury.] [Ariticle in Chinese]

HUANG Xin-Li, ZHOU Xiao-Hong, ZHOU Jun-Lin, DING Chun-Hua, XIAN Xiao-Hui

Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China； Department of Orthopaedics, Beijing Chaoyang Hospital Affiliated to Capital Medical University, Beijing 100020, China

Abstract

The animal model of acute lung injury (ALI) caused by intravenous injection of lipopolysaccharides (LPS) and cultured human peripheral blood polymorphonuclear neutrophil (PMN) were used to study the effects of sodium hydrosulfide (NaHS), hydrogen sulfide (H2S) donor, on LPS-induced PMN accumulation, microvascular permeability and PMN apoptosis. Control group, NaHS group, LPS group and LPS + NaHS group were established both in in vivo and in vitro studies. Microvascular permeability, PMN accumulation in lung and apoptosis of PMN were detected. The results showed that: (1) In in vivo study, PMN accumulation in lung, the protein content in bronchoalveolar lavage fluid (BALF) and the Evans blue dye in lung tissue of LPS group were markedly higher than those of both sham operation group and LPS + NaHS group (P<0.05, P<0.01); (2) In in vitro study, the apoptotic rates of PMN in LPS group and NaHS group were significantly higher than that in control group (P<0.01), while compared with LPS group, LPS + NaHS group showed significantly higher apoptotic rate (P<0.01). These results suggest that NaHS attenuates LPS-induced microvascular permeability and alleviates ALI. PMN apoptosis induced by NaHS is possibly one of the potential mechanisms underlying the decrease of PMN accumulation in lung tissue.

Key words: hydrogen sulfide; endotoxin; acute lung injury; polymorphonuclear neutrophil

收稿日期：2009-03-06　　录用日期：2009-05-15

通讯作者：黄新莉　　E-mail: huangxinli2006@hotmail.com

引用本文：

黄新莉, 周晓红, 周君琳, 丁春华, 羡晓辉1. 中性粒细胞在外源性硫化氢抗内毒素致急性肺损伤中的作用[J]. 生理学报 2009; 61 (4): 356-360.

HUANG Xin-Li, ZHOU Xiao-Hong, ZHOU Jun-Lin, DING Chun-Hua, XIAN Xiao-Hui. [Role of polymorphonuclear neutrophil in exogenous hydrogen sulfide attenuating endotoxin-induced acute lung injury.] [Ariticle in Chinese] . Acta Physiol Sin 2009; 61 (4): 356-360 (in Chinese with English abstract).