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前脑特异性过表达NR2B 基因对小鼠焦虑样行为无明显影响

李世佳, 徐敏华, 李春霞, 周天舒, 孟博, 梅兵

华东师范大学 上海脑功能基因组学研究所； 环境科学系，上海 200062

摘要

N- 甲基-D- 天冬氨酸(N-methyl-D-aspartate, NMDA)受体是一种重要的离子型谷氨酸受体，虽然针对该受体的某些 拮抗剂和部分激动剂在临床上具有抗焦虑的疗效，但NMDA 受体及其各亚基在焦虑样行为中的作用尚不完全明了。本实 验旨在通过观察前脑特异性NR2B 转基因小鼠与对照组小鼠在青年、中年及老年阶段焦虑样行为的差异，探讨NMDA 受体 NR2B 亚基对焦虑样行为的影响，为揭示NMDA 受体在焦虑发生中的作用提供实验资料。结果显示，青年组、中年组和 老年组NR2B 转基因小鼠在开场实验和高架十字迷宫这两个行为学实验中都没有表现出焦虑样行为；毛细管电泳实验证实 NR2B 的高表达也不改变小鼠脑中单胺类神经递质的水平。这些结果初步提示，前脑区NR2B 表达量的提高以及由此导致的 NR1 与NR2A 表达水平的小幅升高对小鼠焦虑样行为无明显影响。

关键词： N R 2 B; 焦虑; 高架十字迷宫; 开场实验; 单胺类神经递质

分类号：Q189

[Forebrain NMDA receptor 2B subunit over-expression has no influence on anxiety behaviors of mice.] [Ariticle in Chinese]

LI Shi-Jia, XU Min-Hua, LI Chun-Xia, ZHOU Tian-Shu, Meng Bo, MEI Bing

Shanghai Institute of Brain Functional Genomics； Department of Environmental Science and Technology, East China Normal University, Shanghai 200062, China

Abstract

It has been known that the glutamate transmission system and N-methyl-D-aspartate receptor (NMDA-R) were possibly related to anxiety processes. Although anxiety symptom can be relieved by NMDA-R antagonists and partial agonists treatment, the functions of NMDA-R and its subunits in anxiety behaviors remain unclear. We used forebrain specific NR2B over-expression mice to examine whether the increase of NR2B subunit level would induce anxiety behaviors. The results indicated that the juvenile (3-5 months old), middle-aged (8-10 months old) and old (19-22 months old ) NR2B transgenic mice showed no significant difference in open field test and elevated plus maze test as compared with the control mice. Capillary electrophoresis of monoamine neurotransmitter in subregions of forebrain revealed no significant difference between transgenic and control mice of 16-18 months age. These results suggest that the increase of NR2B expression and followed NR1 and NR2A expression augmentations in the forebrain have no significant effect on anxiety-related behaviors in mice.

Key words: NR2B; anxiety; elevated plus maze; open field; monoamine neurotransmitter

收稿日期：2009-02-19　　录用日期：2009-04-22

通讯作者：梅兵　　E-mail: bmei@brain.ecnu.edu.cn

引用本文：

李世佳, 徐敏华, 李春霞, 周天舒, 孟博, 梅兵. 前脑特异性过表达NR2B 基因对小鼠焦虑样行为无明显影响[J]. 生理学报 2009; 61 (3): 272-278.

LI Shi-Jia, XU Min-Hua, LI Chun-Xia, ZHOU Tian-Shu, Meng Bo, MEI Bing. [Forebrain NMDA receptor 2B subunit over-expression has no influence on anxiety behaviors of mice.] [Ariticle in Chinese] . Acta Physiol Sin 2009; 61 (3): 272-278 (in Chinese with English abstract).