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钠负荷及依那普利对肾积水小鼠肾素合成与分泌的相互作用

张延玲, 武俊艳, 王学春, 刘磊

泰山医学院基础医学部；泰山学院，泰安 271000

摘要

本文应用肾积水小鼠，探讨了钠负荷及依那普利(enalapril)对肾素合成及分泌的相互作用。实验动物分为4 组：假 手术组、钠负荷组、依那普利组、钠负荷及依那普利联合组。Balb/C 小鼠采用左侧输尿管结扎形成肾积水。在不同的条件 下，对主动脉及双侧肾静脉的血浆肾素浓度(plasma renin concentration, PRC)、肾组织肾素浓度(tissue renin concentration, TRC) 及肾素mRNA水平进行了测定。与假手术组相比，钠负荷小鼠中，双侧肾静脉PRC 降低(P<0.05)，肾积水一侧的TRC 及肾 素mRNA也显著降低(P<0.05)。用依那普利治疗肾积水小鼠后，PRC、双侧TRC 及肾素mRNA水平高于假手术组(P<0.01)。 应用钠负荷及依那普利联合治疗，其效应较单独应用依那普利时为低，肾积水一侧肾静脉PRC 与主动脉内PRC 相似。以上 结果提示，在正常及肾积水肾脏，钠负荷与依那普利通过相互作用而影响肾素- 血管紧张素系统(renin-angiotensin system, RAS)。 肾素合成的调节可独立于致密斑，但后者对肾素分泌起重要调节作用。

关键词： 肾素合成; 钠负荷; 肾积水; 致密斑; 依那普利; 输尿管

分类号：Q463

Interaction of sodium loading and enalapril on renin mRNA and renin releasein hydronephrotic mice

ZHANG Yan-Ling, WU Jun-Yan, WANG Xue-Chun, LIU Lei

Department of Fundamental Medicine； Taishan Medical University； Taishan University, Taian 271000, China

Abstract

The present study investigated the interaction between sodium loading and enalapril on renin synthesis and secretion in hydronephrotic mice. Four different experimental groups (n=10 each) were used: sham-operated animals with normal diet (control group); sodium loading (SL group); enalapril treatment with normal diet (E group), and sodium loading combined with enalapril treatment (SL+E group). The hydronephrotic left kidney was induced by unilateral ureteral ligation in mice in the latter three groups. Plasma renin concentration (PRC) in the aorta, both the left and right renal veins, tissue renin concentration (TRC) and renin mRNA levels in the kidneys were examined under different procedures. In hydronephrotic mice treated with sodium loading, PRC in the left and right renal veins was lower than that in control mice (P<0.05), and TRC and renin mRNA levels in the hydronephrotic kidney were also suppressed (P<0.05). In hydronephrotic mice treated with enalapril, there were significant increases in PRC, TRC and renin mRNA levels in the hydronephrotic and right kidneys compared to the normal control (P<0.01). In hydronephrotic animals treated with sodium loading and enalapril, the increasing response was attenuated, and PRC in the hydronephrotic vein was similar to the level in the aorta. There was an interaction between sodium loading and enalapril on renin-angiotensin system (RAS) in both hydronephrotic and normal kidneys. The mechanism in control of renin synthesis is independent of the macula densa, but the latter is critical in the control of renin secretion.

Key words: renin synthesis; sodium loading; hydronephrosis; macula densa; enalapril; ureter

收稿日期：2009-01-06　　录用日期：2009-05-08

通讯作者：张延玲　　E-mail: zyl@tsmc.edu.cn

引用本文：

张延玲, 武俊艳, 王学春, 刘磊. 钠负荷及依那普利对肾积水小鼠肾素合成与分泌的相互作用[J]. 生理学报 2009; 61 (3): 239-246.

ZHANG Yan-Ling, WU Jun-Yan, WANG Xue-Chun, LIU Lei. Interaction of sodium loading and enalapril on renin mRNA and renin releasein hydronephrotic mice. Acta Physiol Sin 2009; 61 (3): 239-246 (in Chinese with English abstract).