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慢性房颤患者左右心房胶原和基质金属蛋白酶表达差异

许国军, 甘天翊, 汤宝鹏, 李耀东, 郭霞, 阿布力孜·阿布都拉木拉提·阿布提热合曼1，霍 强1

新疆医科大学第一附属医院心脏中心，乌鲁木齐 830054；新疆地方病重点实验室，乌鲁木齐 830054

摘要

本文旨在研究慢性房颤患者左右心房胶原、基质金属蛋白酶(matrix metalloproteinases, MMPs)、基质金属蛋白酶 抑制剂(tissue inhibitors of metalloproteinases, TIMPs)的mRNA水平是否存在差异。选择接受开胸换心脏瓣膜的手术病人共 45 例，其中慢性房颤患者27 例，窦性心律患者18 例；术中分别取左、右心耳组织，采用半定量RT-PCR 法测定I 型胶 原、III 型胶原、基质金属蛋白酶-1，9 (MMP1、MMP9)以及基质金属蛋白酶抑制剂-1 (TMP1)的mRNA 表达水平。结 果显示：(1)与窦性心律组相比，慢性房颤组患者左、右心房肌组织中I 型胶原、MMP1、MMP9 的mRNA 表达水平均 上调(P<0.05)，TMP1 的表达水平均下调(P<0.01)；(2)窦性心律组患者左、右心房肌组之间I 型胶原、III 型胶原、MMP1、 MMP9、TMP1 的mRNA 表达水平的差异均无统计学显著意义(P>0.05)；(3)慢性房颤组患者MMP1 的mRNA 在右心房肌组 织中表达水平较左心房高(P<0.05)，MMP9 的mRNA 在左心房肌组织中表达水平较右心房高(P<0.01)；(4)无论左心房还是 右心房，MMP1、MMP9 的mRNA 表达水平均与I 型胶原的mRNA 表达水平呈正相关，与TMP1 的mRNA 表达水平呈负 相关。以上结果表明，心房肌组织中MMP1/TMP1 以及MMP9/TMP1 的mRNA 表达水平失衡引起I 型胶原转录水平的改 变，可能是慢性房颤患者心房肌间质纤维化的分子基础，在慢性房颤的过程中，MMPs 的mRNA 在左、右心房的表达是 有差异的。

关键词： 心房颤动; 胶原; 基质金属蛋白酶; 基质金属蛋白酶抑制剂

分类号：R541.7

[Differential expression of collagen and matrix metalloproteinases between left and right atria in patients with chronic atrial fibrillation.] [Ariticle in Chinese]

XU Guo-Jun, GAN Tian-Yi, TANG Bao-Peng, LI Yao Dong, GUO Xia, ABUDULA Abulizi

Department of Cardiology, the First Affiliated Hospital of Xinjiang Medical University； Endemic Laboratory of Xinjiang, Urumqi 830054, China

Abstract

The present study aimed to investigate the mRNA expression levels of collagen, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs) in the left and right atria in patients with chronic atrial fibrillation (CAF). Forty five patients with valvular heart disease were sampled in this study, including 18 patients with sinus rhythm (SR), 27 patients with CAF. Clinical data of these patients were collected, and the left and right atrial appendages were obtained from these patients during heart valvular replacement surgery. The mRNA levels of collagen type I, collagen type III, MMP1, MMP9, TMP1 of the atria were then measured by semi-quantitative RT-PCR. The results obtained were as follows. Compared to those in SR group, in atria of CAF group, the mRNA levels of collagen type I, MMP1 and MMP9 increased (P<0.05), while the mRNA level of TMP1 decreased (P<0.01). There were no significant differences in the mRNA levels of collagen type I, collagen type III, MMP1, MMP9 and TMP1 between the left and right atria of SR group (P>0.05). In CAF group, the mRNA level of MMP1 in the right atrium was higher than that in the left atrium (P<0.05), however, the mRNA level of MMP9 in the left atrium was higher than that in the right atrium (P<0.01). In both the left and right atria, the mRNA of collagen type I was positively correlated with the corresponding atrial diameter; the mRNA of MMP1 and MMP9 was positively correlated with the mRNA of collagen type I, and was negatively correlated with the mRNA of TMP1. These results suggest that the increased level of collagen type I associated with selective upregulation of MMP1, 9 and downregulation of TMP1, 9 in the atrium might be the molecular basis of atrial interstitial fibrosis in patients with CAF. Moreover, during CAF development, there is difference in the expression of MMPs between the left and right atria.

Key words: atrial fibrillation; collagen; matrix metalloproteinases; tissue inhibitors of metalloproteinases

收稿日期：2009-02-23　　录用日期：2009-05-04

通讯作者：汤宝鹏　　E-mail: tangbaopeng@hotmail.com

引用本文：

许国军, 甘天翊, 汤宝鹏, 李耀东, 郭霞, 阿布力孜·阿布都拉木拉提·阿布提热合曼1，霍 强1. 慢性房颤患者左右心房胶原和基质金属蛋白酶表达差异[J]. 生理学报 2009; 61 (3): 211-216.

XU Guo-Jun, GAN Tian-Yi, TANG Bao-Peng, LI Yao Dong, GUO Xia, ABUDULA Abulizi. [Differential expression of collagen and matrix metalloproteinases between left and right atria in patients with chronic atrial fibrillation.] [Ariticle in Chinese] . Acta Physiol Sin 2009; 61 (3): 211-216 (in Chinese with English abstract).