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龙血素B 诱导大鼠初级感觉神经元胞内Ca2+ 浓度缓慢上调

杨一宁, 陈珏旭, 庞雪雁, 寺川进, 陈旭, 吉永华, 雍克岚

上海大学神经药理毒理实验室，上海 200444； 滨松医科大学光量子研究中心，滨松431-3192，日本

摘要

本研究采用Fura-2 荧光染色的方法，在急性分离的背根神经节(dorsal root ganglia, DRG)神经元细胞上，观察了由传 统中药龙血竭活性成分龙血素B 诱导的[Ca2+]i 的变化。在DRG 细胞上施加龙血素B 引起[Ca2+]i 的上升，且存在剂量依赖效 应。同样的结果出现在细胞外液无钙的情况，但此时[Ca2+]i 上升幅度远远小于在标准细胞外液情况下出现的上升幅度。这表 明DRG 的[Ca2+]i 的升高主要是由细胞外Ca2+ 内流而产生的，而不是由细胞器如线粒体和胞内钙库释放Ca2+ 产生的。另外，咖 啡因和龙血素B 共孵育也可以诱导[Ca2+]i 的升高，但是其上升幅度显著小于单独施加咖啡因时的幅度，说明龙血素B 与咖啡 因作用的通路或靶器有所重叠。与咖啡因、高钾和辣椒素诱导的瞬变相比，龙血素诱导的DRG 的[Ca2+]i 的上升更加缓慢且 持续时间较长。这些结果提示，龙血素B 诱发的初级感觉神经元[Ca2+]i 的升高是稳定且独特的。

关键词： 龙血素B ; 背根神经节; 胞内钙离子; 咖啡因; Fura-2

分类号：Q189

Slow rise of intracellular Ca2+ concentration in rat primary sensory neurons triggered by loureirin B

YANG Yi-Ning, CHEN Jue-Xu, PANG Xue-Yan, TERAKAWA Susumu, CHEN Xu, JI Yong-Hua, YONG Ke-Lan

Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, China； Photonmedical Research Center, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan

Abstract

In the present study, the intracellular free calcium concentration ([Ca2+]i) in acutely isolated rat dorsal root ganglia (DRG)
neurons modulated by loureirin B, an active component of “dragon’s blood” which is a kind of Chinese herbal medicine, was determined
by the means of Fura-2 based microfluorimetry. It was found that loureirin B could evoke the elevation of [Ca2+]i in a dose-dependent
manner. However, the elevation of [Ca2+]i evoked in the calcium free solution was much smaller than that in the standard external cell
solution, suggesting that most change of [Ca2+]i was generated by the influx of extracellular Ca2+, not by the activities of intracellular
organelles like Ca2+ stores and mitochondria. In addition, the mixture of loureirin B and caffeine also induced [Ca2+]i rise, but the peak
of [Ca2+]i rise induced by the mixture was significantly lower than that by caffeine alone, which means the triggering pathway and the
targets of caffeine are probably involved in loureirin B-induced [Ca2+]i rise. Moreover, compared to the transients induced by caffeine,
KCl and capsaicin, the loureirin B-induced [Ca2+]i rise is much slower and more stable. These results indicate that the capability of
loureirin B of inducing the [Ca2+]i rise is solid and unique.

Key words: loureirin B; dorsal root ganglia neurons; [Ca2+]i; caffeine; Fura-2

收稿日期：2008-08-29　　录用日期：2009-02-05

通讯作者：吉永华,雍克岚　　E-mail: yhji@staff.shu.edu.cn, klyong@staff.shu.edu.cn

引用本文：

杨一宁, 陈珏旭, 庞雪雁, 寺川进, 陈旭, 吉永华, 雍克岚. 龙血素B 诱导大鼠初级感觉神经元胞内Ca2+ 浓度缓慢上调[J]. 生理学报 2009; 61 (2): 115-120.

YANG Yi-Ning, CHEN Jue-Xu, PANG Xue-Yan, TERAKAWA Susumu, CHEN Xu, JI Yong-Hua, YONG Ke-Lan. Slow rise of intracellular Ca2+ concentration in rat primary sensory neurons triggered by loureirin B. Acta Physiol Sin 2009; 61 (2): 115-120 (in Chinese with English abstract).