过刊浏览

低氧预适应小鼠皮层Bcl--2和Caspase--3的表达变化

张颜波, 吕国蔚, 杨明峰, 牛敬忠, 孙保亮

泰山医学院附属医院神经内科.山东,泰安 271000；首都医科大学低氧医学研究所.北京 100069

摘要

该文探讨小鼠皮层Bcl--2和Caspase--3在低氧预适应脑保护中的作用。将Blb/c近交系小鼠随机分为对照组、低氧组和低氧预适应组，用免疫荧光和激光共聚焦显微镜等技术测定皮层顶叶Bcl--2和Caspase--3表达荧光强度和阳性细胞计数。结果显示，低氧组和低氧预适应组Bcl--2表达均显著高于对照组，低氧预适应组又显著高于低氧组。低氧组和低氧预适应组Caspase--3表达均显著高于对照组，但低氧预适应组显著低于低氧组。结果表明，低氧预适应过程中，小鼠皮层脑区通过Bcl--2高表达和Caspase--3低表达，抵御皮层细胞凋亡，参与脑保护机制。

关键词： 低氧预适应; Bel-2; caspase-3; 凋亡; 皮层; 小鼠

Changes in Bcl--2 and Caspase--3 expressions in cortex of hypoxic preconditioning mice

Zhang Yanbo, Lu Guowei, Yang Mingfeng, Niu Jingzhong, Sun Baoliang

Department of Neurology, Affiliated Hospital of Taishan Medical College.Tai'an 271000,Shandong；China

Abstract

The purpose of the present study was to explore the roles of Bcl-2 and Caspase-3 in mouse cortex in hypoxic preconditioning.Blb/c mice were randomly divided into three groups: control group, hypoxic group and hypoxic preconditioning group. Fluorescence intensity of Bcl-2 and Caspase-3 was observed and number of positive cells was counted in parietal cortex by immunofluorescence andconfocal laser scanning microscope. Fluorescence intensity of Bcl-2 in the normal group, hypoxic group and hypoxic preconditioning group was 6.2±1.7, 68.5±13.1,180.6±34.8, respectively, and number of Bcl-2-positive cells was 18.5±4.9, 52.3±10.5, 150.8±24.7,respectively. Fluorescence intensity of Caspase-3 in the control group, hypoxic group and hypoxic preconditioning group was 8.6±2.0, 40.2±8.2, 26.4±6.1, respectively, and number of Caspase-3-positive cells of was 4.3±1.2, 63.6±12.5, 45.7±9.8, respectively. Theresults showed that the expressions of Bcl-2 in both hypoxic group and hypoxic preconditioning group were significantly higher thanthat in the control group; and the expression of Bcl-2 in hypoxic preconditioning group was even higher than that in hypoxic group. The expressions of Caspase-3 in hypoxic group and hypoxic preconditioning group were also significantly higher than that in the control group; whereas the expression of Caspase-3 in hypoxic preconditioning group was significantly lower than that in hypoxic group.These results suggest that cortex cells are resistant to apoptosis via increased expression of Bcl-2 and lowered expression of Caspase-3 in the cortex and brain cells are thereby protected during hypoxic preconditioning.

Key words: Hypoxic preconditioning;Bcl-2;Caspase-3;Apoptosis;Cortex;Mice

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

张颜波, 吕国蔚, 杨明峰, 牛敬忠, 孙保亮. 低氧预适应小鼠皮层Bcl--2和Caspase--3的表达变化[J]. 生理学报 2008; 60 (2): 249-253.

Zhang Yanbo, Lu Guowei, Yang Mingfeng, Niu Jingzhong, Sun Baoliang. Changes in Bcl--2 and Caspase--3 expressions in cortex of hypoxic preconditioning mice. Acta Physiol Sin 2008; 60 (2): 249-253 (in Chinese with English abstract).